Flashcards
CHEM 2305: HPLC (Notes # 6)
Cards in this deck (21)
- 1HPLC = ?High Performance Liquid Chromatography
https://en.wikipedia.org/wiki/High-performance_liquid_chromatography - 2GC = ?Gas chromatography
https://en.wikipedia.org/wiki/Gas_chromatography - 3HPLC vs. GC Sample must be.........HPLC: volatile
GC: soluble in the mobile phase - 4HPLC vs. GC Temperature.........HPLC: sample must be able to survive high injection temperature
GC: can be operated around room temperature - 5HPLC vs. GC Sample preparation..........HPLC: very little sample prep required
GC: sample must be filtered - 6HPLC vs. GC mobile phase.......... mobile phase....HPLC: generally has no effect on separation
GC: stationary phase and mobile phase take part
HPLC: mobile phase a liquid
GC: mobile phase a gas - 7HPLC vs. GC detector..........HPLC: FID
GC: UVVis/Fluorescence
REfractive Index Conductivity for ion exchange????? - 8In general, HPLC is used for NON-POLAR/POLAR GC is used for NON-POLAR/POLARHPLC = polar
GC = non-polar - 9polarity ranges from: from _______ (least polar) to __________ (most polar)hydrophobic (least polar)
hydrophilic (most polar) - 10volatility ranges from: from _______ (least volatile) to __________ (most volatile)non-volatile (least volatile)
volatile (most volatile) - 11In general, HPLC is used for NON-VOLATILE/VOLATILE GC is used for NON-VOLATILE/VOLATILEHPLC = non-volatile
GC = volatile - 12sdfsfsdfsdf
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- CHEM 2305: HPLC (Notes # 6) Flashcards on CHEM 2305: HPLC (Notes # 6), created by Monika H on 29/10/2016.
- Created by
- Monika H · Oct 2016
- Updated
- Oct 30, 2016
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