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Flashcards

Microbiology Lab exam #3

ski_bumb English 150 cards

Cards in this deck (150)

  1. 1What does API test formembers of the family Enterobacteriaceae
  2. 2ONPG test for?Fermentation of lactose
    galactose permease
  3. 3positive ONPGyellow
  4. 4negative ONPGcolorless
  5. 5What does ADH test?Decarboxylase
    (removes amino acid)
    Arginine
  6. 6ADH positivered/orange
  7. 7ADH negativeyellow
  8. 8what does LDC testDecarboxylase
    (removes amino acid)
    Lysine
  9. 9positive LDCred/orange
  10. 10negative LDCyellow
  11. 11what does OCD testDecarboxylase
    (removes amino acid)
    Ornithine
  12. 12Positive OCDred/orange
  13. 13negative OCDyellow
  14. 14what does CIT test?Citrate as a carb source
    Citrate permease
  15. 15positive CITblue
  16. 16negative CITgreen
  17. 17what does H2S test?Thiosulfate reductase
  18. 18positive H2Sblack
  19. 19negative H2Sclear
  20. 20What does URE test?Urease
  21. 21positive UREred/orange
  22. 22negative UREyellow
  23. 23What does TDA test?Tryptophan deaminase
  24. 24positive TDAdark brown
  25. 25negative TDAno dark brown
  26. 26What does IND test?Tryptophanase
  27. 27positive INDred
  28. 28negative INDyellow
  29. 29What does VP test?Voges Proskauer
    Alcohol fermentation
  30. 30Positive VPred
  31. 31negative VPcolorless/faint pink
  32. 32what is the second TDA reagent?10% Ferric chloride
  33. 33What is the second reagent added to IND?James reagent
  34. 34What is the second reagent added to VP?VP1 & VP2
  35. 35What does GEL test?Gelatinase
  36. 36Positive GELblack throughout
  37. 37negative GELblack only in beads
  38. 38What does GLU test?Glucose fermentation
  39. 39positive GLUyellow
  40. 40negative GLUblue/green
  41. 41What does MAN test?Mannitol fermentation
    glucose
  42. 42positive MANyellow
  43. 43negative MANblue/green
  44. 44what does INO test?Inositol fermentation
    glucose
  45. 45positive INOyellow
  46. 46negative INOblue/green
  47. 47What does SOR test?Sorbitol fermentation
    glucose
  48. 48Positive SORyellow
  49. 49Negative SORblue/green
  50. 50What does RHS test?Rhamnose Fermentation
    glucose
  51. 51Positive RHAyellow
  52. 52negative RHAblue/green
  53. 53What does SAC test?Sucrose fermentation
    glucose
  54. 54Positive SACyellow
  55. 55Negative SACblue/green
  56. 56What does MEL test?Melibiose fermentation
  57. 57Positive MELyellow
  58. 58Negative MELblue/green
  59. 59What does AMY test?Amygdalin fermentation
    glucose
  60. 60positive AMYyellow
  61. 61negative AMYblue/green
  62. 62What does ARA test?Arabinose fermentation
    glucose
  63. 63Positive ARAyellow
  64. 64Negative ARAblue/green
  65. 65First part of broth testAdd 2 drops of Nit 1 and Nit 2, if red appears +
  66. 66Second part of Nitrate testIf 1st part is clear add pinch of zinc(nit C) if + if red, - if clear
  67. 67Catalase positivebubbles
  68. 68Catalase negativeno bubbles
  69. 69How to perform Oxidase testSwab sample, add 1 drop of oxidase reagent to swab
  70. 70positive Oxidase testblue/black right away
  71. 71negative Oxidase testno change or change after 30 sec.
  72. 72What is Tetracycline mechanism of actionInhibit protein synthesis
  73. 73What is Gentamicin mechanism of actionInhibit protein synthesis
  74. 74Is Tetracycline broad or narrowBroad
  75. 75Is Gentamicin broad or narrow?Broad
  76. 76What is Erythromycin mechanism of action?Inhibit protein synthesis
  77. 77Is Erythromycin Broad or narrowEffective against G+ & a few G-
  78. 78What is Chloramphenicol mechanism of action?Inhibit protein synthesis
  79. 79Is Chloramphenicol broad or narrow?Broad
  80. 80What is Streptomycin mechanism of action?Inhibit protein synthesis
  81. 81Streptomycin broad or narrowBroad
  82. 82What is Polymyxin mechanism of action?Destroys cytoplasmic membranes
  83. 83Is Polymyxin broad or narrowNarrow
  84. 84What is Rifampin mechanism of actionInhibits nucleic acid synthesis (mRNA)
  85. 85Is Rifampin broad or narrowNarrow
    Aerobic G+ & mycobacteria
  86. 86What is Quinolone mechanism of actionInhibits nucleic acid synthesis (DNA)
  87. 87Quinolone broad or narrowNarrow
    Aerobic G+ & mycobacteria
  88. 88What is Penicillin mechanism of actionImpairs cell wall synthesis
  89. 89Is Penicillin broad or narrowNatural=narrow
    semisynthetic= broader
  90. 90Sulfas drugs mechanism of actionCompetative Inhibition (block enzymes)
  91. 91What do Sulfa drugs act on?Block synthesis of folic acid
  92. 92What are the classes of antibiotics?Natural
    Semi-synthetic
    Synthetic
  93. 93What are Natural antibiotics?Produced by organisim
  94. 94What are Semi-synthetic antibiotics?Made by organisim then modified
  95. 95What are synthetic antibiotics?Made in lab
  96. 96What are the degrees of disinfectant control?Sterilization, Disinfection, Sanitization, Degermation, Preservation, Microbicidal
    Microstatic
  97. 97What is sterilization?Removing/killing all microbes & spores
  98. 98What is disinfection?Killing all vegetative cells (not spores)
  99. 99What is disinfectanton inanimate objects
    Bleach
  100. 100What is antisepticdisinfect living tissue
    Listerine
  101. 101What is Sanitization?Reducing the # of microbes on inanimate objects
  102. 102What is degermination?Reducing the # of microbes on living tissues
  103. 103What is preservation?Preventing microbial growth to inhibit deterioration on non-edibles or food
  104. 104What is microbicidal?Kills microbes
  105. 105Example of a microbicidalbacteriocidal, fungicidal
  106. 106What is microbistatic?inhibits growth of microbes
  107. 107microbistatic examplebacteriostatic, fungistatic
  108. 108Methods of bacterial controlPhysical & Chemical
  109. 109Types of Physical bacterial controlTemp., Radiation, Osmotic pressure, Dessication, Sonic vibration, filtration
  110. 110What is an example of Temperature bacterial controlIncineration w/ Bunsen burner
  111. 111What is example of radiation bacterial controlUV light
  112. 112What is Sonic vibration bacterial controlCells shaken apart
  113. 113What is Filtration bacterial controlPass liquid through a filter having very tiny holes
  114. 114What are chemical means of bacterial controlChemotherapeutic agents (antibiotic)
    Disinfectants
  115. 115What do chemotherapeutic agents do?Treat infectious diseases
  116. 116What so disinfectants do?Prevent contamination and infection
  117. 117What are the classes of disinfectants?Alcohols, Halogens, Peroxides, Aldehydes, Phenolics, Surface active agents
  118. 118What do alcohols do?Denature proteins, dilute up to 70%, Made into tintures
  119. 119what is a tincture?When mixed w/other chemicals
  120. 120What is an example of a tincture?Tincture of benzoic
  121. 121What do Halogens do?Oxidize biomolecules
  122. 122Example of a Halogen?Chlorine & Iodine
  123. 123What are the 2 forms of chlorine as a halogen?In form of Cl2 gas & in form of OCl- (hypochlorite)
  124. 124How is Cl2 gas used?To sterilize things that are heat sensitive
  125. 125Example of OCl-bleach
  126. 126Examples of IodineIodophore & betadine (iodine + surface-active agent)
  127. 127What do Peroxides doOxidize biomolecules
  128. 128What is class of disinfectants is detoxified by catalasePeroxides
  129. 129What is given off when Peroxides are detoxified02
  130. 130What do Aldehydes do?Coagulate protiens
  131. 131Examples of Aldehydesformaldehyde & glutaraldehyde
  132. 132What do Phenolics do?Destroy cell membranes
    Related to Phenol
  133. 133Examples of PhenolicsLysol, hexachloraphene(phisohex), triclosan
  134. 134What do surface-active agents (surfactants) do?disrupt cell membrane
  135. 135What class of disinfectants are amphipathic?Surface-active agents (surfactants)
  136. 136What is AmphipathicHave hydrophilic & hydrophobic portions
  137. 137Examples of surface-active agentssoaps, emulsifiers, detergents
  138. 138What is Immunofluorescencespecific antibodies, labeled w/a fluorescent dye, are used to identify a particular microbe
  139. 139What do u see when an immunofluorescence side is exposed to UV lightOmits green light
  140. 140What is Quenching?Fading of the glowing of the cells
  141. 141what is direct immunofluorescenceFluorescent labeled antibodies bind directly to microbe of intrest
  142. 142what is indirect immunofluorescenceFluorescent labeled antibodies bind to antibodies formed d/t presence of microbe
  143. 143Did we perform direct or indirect Immunofluorescence?Direct
  144. 144What fluorescent dye did we use?Fluorescein
  145. 145What does ELISA stand for?Enzyme Linked Immunosorbent Shift Assay
  146. 146What is an indirect ELISA?tests for HIV & rubella
  147. 147What is a direct ELISA test?Prego test
  148. 148how is ELISA performedprimary AB is present it binds to antigen, 2nd AB recognizes pAB, if 2AB present it turns brown
  149. 149What was the secondary antibody used in our ELISA test?HRP (horseradish peroxidase) covalently attached
  150. 150Be able to sketch how to perform ELISA testdraw it out

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Flashcards on Microbiology Lab exam #3, created by ski_bumb on 20/04/2014.
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ski_bumb
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