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DISAGGREGATION OF PLANTS
1.PRIMARY EXPLANT DIAGGREGATION.
2.MECHANICAL DISAGGREGATION.
3.ENZYMATIC DISAGGREGATION.
4.EDTA TREATMENT.
DISSECTION.
FINE CHOPPING
WASH BY SETTING.
TRANSFER IN LAST WASH.
ADD THIN FILM OF MEDIUM.
INCUBATE FOR 2-3 WEEKS
REMOVE BBS
EXPLANT OUTGROWTH
TISSUE SAMPLE
CELL+TISSUE DEBRIS
MORE CELLS + TISSUE DEBRIS
VIABLE CELLS+TISSUE DEBRIS
VIABLE CELLS +DAMAGED CELLS
PRIMARY ADHERENT CELL CULTURE.
BLOPSY IN MEDIUM + ANTIBIOTIC
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- Flowchart on Untitled, created by Akshaya Akshaya on 03/01/2018.
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- Akshaya Akshaya
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