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Flowchart

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Jaya Deepa English

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  1. DISAGGREGATION OF EXPLANT

  2. mechanical disaggreation

  3. primary explant techinque

  4. enzymatic disaggreation

  5. dissection

  6. tissue sample

  7. cell + tissue debris

  8. biopsy in medium in antibiotic

  9. fine chopping

  10. chopped tissue

  11. wash by settling

  12. transfer in last wash

  13. more cell +tissue debris

  14. viable cell +tissue debris

  15. complete medium collagenase

  16. dissaggerated tissue tissue residues

  17. remove bss

  18. viable cell +damaged cell

  19. dispersed cell +resiudal culture

  20. add thin flim medium

  21. primary adherant cell culture

  22. primary cell culture

  23. primary cell culture

  24. incubate for 2_3 weeks

  25. explant ou

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Flowchart on Untitled, created by Jaya Deepa on 04/01/2018.
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Jaya Deepa
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