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Flowchart

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Salma Jaffar English 5.0Average rating 5.0 out of 5 (1 rating)

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  1. STEPS INVOLVED IN CELL CULTURE

  2. Disaggregation of Explant

  3. PRIMARY EXPLANT TECHNIQUE

  4. MECHANICAL DISAGGREGATION

  5. ENZYMATIC DISAGGREGATION

  6. EDTA TREATMENT

  7. Dissection

  8. Fine chopping

  9. Wash by settling

  10. Tissue sample

  11. Cell + debris

  12. More cell +debris

  13. Viable cell+debris

  14. Chopped

  15. Washed

  16. Cold trypsinization

  17. Tissues in vials

  18. Collagenase

  19. Centrifuge

  20. Resuspend

  21. Wash primary cell culture

  22. Warm trypsinization

  23. Transfer to BSS

  24. Remove to BSS

  25. Dilute the cells

  26. Add thin film of medium

  27. Incubate for 2-3 weeks

  28. Incubate

  29. Primary adherent cell culture

  30. Explant out growth

  31. Place on ice for 1.6 hrs

  32. Disaggregate cells+ tissue residues

  33. Incubate 335.6 degree 20-30 min

  34. Tissue pieces+ 100ml trypsin

  35. Pieces +trypsin

  36. Dispersed cells in medium

  37. Incubate 35.6 degree for 48-72 hrs

  38. Dispersed cells

  39. Primary cell

  40. Subculture

  41. Cell line

Information

Description
Flowchart on Untitled, created by Salma Jaffar on 08/01/2018.
Created by
Salma Jaffar
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Average rating 5.0 out of 5 (1 rating)

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