DNA Sequencing- SANGER Method

Description

AS level Biology (Module 6- Genetics and Ecosystems) Flowchart on DNA Sequencing- SANGER Method, created by 007842-Stuart Denton on 30/05/2017.
007842-Stuart Denton
Flowchart by 007842-Stuart Denton, updated more than 1 year ago
007842-Stuart Denton
Created by 007842-Stuart Denton about 8 years ago
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Resource summary

Flowchart nodes

  • DNA sequence is amplified- Via cloning the DNA
  • Using heat the DNA helix is split into template and complementary strand
  • Primer is added to the 5' end of the DNA template
  • DNA strands added to four test tubes
  • All four of the Free nucleotides are added to solution
  • Specially Modified DNA nucleotides are added to test tubes separately
  • DNA Polymerase is added to solution
  • DNA polymerase binds to primer and works in 5' to 3' direction binding nucleotides
  • Until specialised DNA nucleotide is reached and sequence terminates.
  • Test tubes added to gel, ELECTROPHORESIS splits the varying sequence lengths
  • Sequences move from cathode to anode, because of the negative phosphate backbone.
  • Lighter, shorter sequences move further.
  • DNA is read from the bottom up, producing complementary strand of DNA
  • Amplified
  • Heat, Split
  • Primer
  • Four Test Tubes
  • Free Nucleotides
  • DNA Modification
  • DNA Polymerase
  • Binding Nucleotides
  • Sequence Terminates
  • Electrophoresis
  • Negative Phosphate backbone
  • Lighter, Shorter sequence
  • Bottom Up
  • Complementary strand of DNA
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