Soil contains mineral particles of different sizes. In a sandy soil
the particles are smaller than in a clay soil
Loam is a soil that contains a mixture of clay and sand, and a large
amount of partly decomposed animal and plant waste called humus
Simple experiments can be performed on different soil samples to compare the contents. Humus content can be found by
burning off the humus using a Bunsen burner. Air content can be found by seeing how much water is needed to fill the air
spaces. Water content can be found by slowly heating the soil to evaporate the water
If a soil has larger particles, then the air content and
permeability is usually higher. If a soil has larger amounts
of humus it will often hold more water and air
Living in Soil
Many organisms live in soil and depend on a supply of oxygen for respiration
and water for chemical reactions. These organisms form many food webs
Humus in the soil is important to living organisms
because it will decompose to release minerals, and
increase the air content of the soil
Earthworms are also important to soil structure and fertility because they bury
organic material for decomposition by bacteria and fungi, aerate and drain the
soil, mix up soil layers and neutralise acid soil
The aeration and draining produced by earthworms will allow organisms to respire aerobically
Neutralising acid soils is important because some plants will not grow if
the pH is too low and mixing up soil layers is important so that dead
material is decomposed
Many of these important functions of earthworms were first understood by Charles Darwin
Microscopic Life in Water
Advantages and Disadvantages of Living in Water
Living in water has a number of advantages. There is no risk of water shortage and
dehydration. The temperature of the water varies less than air temperature. Water helps
provide support. Waste product are easily disposed of into the water
It also has some disadvantages. The water content of the body can vary
and needs to be controlled. Water is denser than air and so resist
movement
If the water is freshwater, then organisms can take up too much water by osmosis. In salt water,
too much water may be lost to the surroundings by osmosis
Organisms such as amoeba have a contractile vacuole that can store any excess water. The vacuole can then fuse
with the cell membrane and empty the water to the outside
Variations in the Numbers of Organisms
The numbers of phytoplankton (tiny aquatic plants)
and zooplankton (tiny aquatic animals) vary at
different depths and in different seasons
This is because factors that affect the photosynthesis of phytoplankton will vary. There will be less light in winter
and deeper in the water. The temperature will be lower in winter and deeper in the water. Minerals are used up
towards the end of summer.
Food webs of marine organisms can provide useful information. The webs
rely directly on different sources of food. Most rely directly on green plants.
others deeper in the ocean feed on dead material called marine snow that
floats down. Some rely on bacteria, deep in the ocean, acting as producers
Water Pollution
Sewage and fertiliser run-off can cause a process called eutrophication to occur. This involves the
rapid growth of algae, which then all die and decay. This uses up oxygen, causing the death of animals
because they are unable to respire
Some species of organisms are more sensitive to pollution than
others and so they can be used as biological indicators for pH and
oxygen.
Some chemicals such as PCBs and DDT can kill animals at the top of marine
food chains. This is because the chemicals: are toxic, do not break down
quickly and so accumulate and become concentrated higher up the food chain,
and affect animals with a long lifespan, such as whales
Enzymes in Action
Enzymes in Washing Powder
Biological washing powders often use enzymes such as:
amylase, to digest carbohydrates such as starch, lipase, to digest
fat and remove fatty stains, and protease, to digest fat and
remove fatty stains
Biological washing powders work best at moderate
temperatures because this is the optimum temperature for
enzymes to work
After treatment with enzymes, the products of digestion are soluble and so
will easily wash out of the clothes
Biological washing powders may not work in acidic or alkaline tap
water because this is not the optimum for the enzymes and they
might start to denature
Enzymes and Sweeteners
Sucrose can be broken down by the use of an enzyme called sucrose (invertase)
When sucrose is broken down by enzymes, the product is much
sweeter, allowing the food industry to use less in food products
Invertase converts sucrose into glucose and fructose
Glucose and fructose are sweeter than sucrose, so less has to be added to the food,
lowering the cost and the energy content
Lactase and Immobilised Enzymes
Enzymes can be immobilised in gel beads by mixing the enzyme with alginate, and
dropping the mixture into calcium chloride solution
The immobilised enzymes produced are very useful in reactions. This is because the
mixture does not become contaminated with the enzyme and they can be used in
continuous flow processing
Some people or animals are lactose intolerant because
they cannot produce the enzyme lactase. This means
that bacteria in the gut ferment lactose, which produces
diarrhoea and wind
Milk can be treated for people who have lactose intolerance. Immobilised lactase
is used to convert lactose in milk into glucose and galactose. Glucose and
galactose can then be absorbed from the milk with no side effects
Gene Technology
Principles of Genetic Engineering
Genetic engineering involves transferring a gene from
one organism to another. The organism that receives the
new gene is called a transgenic organism
The main stages in genetic engineering involve identifying and
removing a desired gene from one organism, cutting open the DNA
in another organism, inserting the new gene into the DNA and
making sure that the gene works in the transgenic organism
The cutting and inserting of DNA is achieved
using enzymes and often the transgenic
organism can be cloned to produce identical
copies
The process of genetic engineering works because
the genetic code is universal. This means that
genes from one organism will produce the same
protein in another organism
Restriction enzymes are used to cut open DNA. They leave several unpaired
bases (single strands) on the cut end. This acts as a 'sticky end'.
Ligase enzymes will join DNA strands because the 'sticky ends' on each cut section of
DNA can join by complementary base pairing
Genetically Engineering Bacteria
Bacteria can be used in genetic engineering to produce human insulin. This
involves cutting the gene for producing human insulin out of human DNA, cutting
open a loop of bacterial DNA, inserting the insulin gene into the loop, and
inserting the loop into a bacterium
Many copies of the bacteria are cultured by cloning and large quantities of insulin are harvested
The loops of DNA used in this process are called plasmids. They are found in the cytoplasm of bacteria and
because the can be taken up by bacteria, they can be used as vectors for genes
To find out whether a bacterium has taken up a plasmid, an assaying technique is used.
Scientists add genes that make the bacteria resistant to antibiotics. The bacteria are then
flooded with the antibiotic by being grown on nutrient agar containing the antibiotic.
Scientists then choose the bacteria that survive
DNA Fingerprints
DNA 'fingerprints' can be produced to identify individuals. They can be stored to help identify people who commit
crimes and prove the innocence of others. However, some people are worried that they may be used for a
variety of other reasons, such as assessing the likelihood of a person developing a disease. The information
could be used as a reason to withhold life insurance
The stages in the production of a DNA 'fingerprint' include extracting DNA forma
sample, such as blood, cutting up or fragmenting the DNA using restriction enzymes,
separating the fragments using electrophoresis, and making the fragments visible
using a radioactive probe