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Chromatography

julie048 English

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Chromatography

  1. Technique 4 separation/identification of molecules based on differential affinity

    • Can be used for qualitative testing
      • Qualitative- how much substance you have
        • Differential Affinity - to attach to something (weak Bond)
          • Strong Bond - are Stable
            • Weak Bond- Hydrogen- Hydrophobic = Flexibility
              • DNA-2 Strands= Flexible
        • 2 Phases of Chromatography
          • 1st- Stationary (Cellulose paper) can be known/unknown
            • 2nd- Mobile ( Liquid) Solution will pass over stationary
              • Separation = Decanting
                • Each samples has their own differential affinity
                  • Affinity can identify chemical and allows you to identify them as well
                    • Capillary Action- movement of fluid, up thin glass
                    • Adhesive- unlike molecules
                      • Cohesive- same molecules
                        • Water has both Cohesive/Adhesive properties
                  • Cohesive Color moves up together
                    • Colors adhere to paper

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Description
Mind Map on Chromatography, created by julie048 on 30/09/2013.
Created by
julie048 · Sep 2013
Updated
Feb 19, 2016
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