TYPES OF CHROMATOGRAPHY

TYPES OF CHROMATOGRAPHY

1. BASED ON THE SHAPE OF CHROMATOGRAPHIC BED

   • COLUMN CHROMATOGRAPHY It is Used to isolation metabolites from biological fluids
   • PLANAR CHROMATOGRAPHY
     • PAPER CHROMATOGRAPHY It is Used in DNA and RNA fingerprinting.
     • THIN LAYER CHROMATOGRAPHY It is used to separate non-volatile mixtures.

2. BASED ON THE MECHANISM OF SEPARATION

   • PARTITION CHROMATOGRAPHY is used to separate and identify proteins, nucleic acids, sugars, glycosides, lipids, alkaloids, and other biomolecules.
   • ADSORPTION CHROMATOGRAPHY It is used for separating unsaturated hydrocarbons from the mixture of organic compounds.
   • SIZE EXCLUSION CHROMATOGRAPHY It is used for the purification of biological macromolecules.
   • AFFINITY CHROMATOGRAPHY It is used in nucleic acid purification, protein purification from cell-free extracts
   • ION EXCHANGE CHROMATOGRAPHY It can be used for the separation and purification of many charged or ionizable molecules such as proteins, peptides, enzymes, nucleotides, DNA, antibiotics, vitamins

3. BASED ON PHYSICAL STATE OF MOBILE/STATIONARY PHASE

   • GAS CHROMATOGRAPHY
     • GAS-LIQUID CHROMATOGRAPHY It is used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition
     • GAS-SOLID CHROMATOGRAPHY I is used for separation of hydrogen isotopes and isomers to high boiling point substances.
   • LIQUID CHROMATOGRAPHY
     • HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY HPLC is applied for molecular weight determination
     • REVERSE PHASE CHROMATOGRAPHY analytical separation of drugs and metabolites as well as extraction of various contaminants in environmental samples.

4. SUPERFICIAL FLUID CHROMATOGRAPHY It is used for achiral separations and purifications in the pharmaceutical industry.