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Quantal Analysis
- Is it a Pre-S change?
- Coefficent of Variance
- If low P(r) then variance about the mean will be high - more scope for fluctuations
- Therefore a decrease in the CV indicates an increase in P(r)
- Therefore a decrease in the CV indicates an increase in P(r)
- No change in CV = no change in P(r) = post S mech
- But SILENT SYNAPSES - if LTP un-silences a synapse, then CV will increase with LTP, even though no change in P(r) thus can still be a post-s mechnism
- If low P(r) then variance about the mean will be high - more scope for fluctuations
- Blockade of NMDArs
- Apply MK-801 -use-dependent, irreversible NMDAr blocker
- Can measure how quickly the NMDAr is blocked
- Higher rate of block = increase in P(r) = pre S mechanism
- Can measure how quickly the NMDAr is blocked
- Apply MK-801 -use-dependent, irreversible NMDAr blocker
- Liu and Tsien (1990)
- Coefficent of Variance
- Synaptic efficacy
- Size of response every time you get one - failures and successes
- time-averaged Post-S response
- time-averaged Post-S response
- Size of response every time you get one - failures and successes
- Synaptic potency
- Average size of response when there is one
- Average size of response when there is one
- If P(r) = 1 then potency = efficacy
- If P(r)< 1 then efficacy<potency
- If P(r)< 1 then efficacy<potency
- P(r) studies
- Minimal stimulation + patch clamp recording
- Do paired pulse stimulation and can measure No. of FAILURES!!
- If rate of failures changes then P(r) must change - preS mechanism
- If rate of failures changes then P(r) must change - preS mechanism
- Do paired pulse stimulation and can measure No. of FAILURES!!
- Minimal stimulation + patch clamp recording
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