Biochemistry

Biochemistry

1. Biurt test

   • 3 ml protein+ 1 drop CuSo4 + 1 ml NaOH
     • Violet color
       • protein
     • blue color
       • carbohydrate
         • Molish test
           • 3 ml unkonw + 2-4 drops a-naphtol + H2So4
             • Violet ring
               • carbohydrate
                 • Iodine test
                   • 10 ml carbohydrate + 2-4 iodine drops
                     • violet
                       • dextrin
                         • fehling test
                           • FA + 1 ml FB ---?shake + 2 ml carbohydrate + heat
                             • partial red ppt
                               • dextrin
                             • red ppt
                               • glucose or fructose
                                 • seliwanoff test
                                   • 1/2 ml carbohydrate + 5 ml seliwanoff reagent + heat
                                     • red color
                                       • fructose or sucrose
                                     • no red
                                       • glucose
                             • no red ppt
                               • sucrose or starch
                                 • seliwanoff test
                                   • /2 ml carbohydrate + 5 ml reagent + heat
                                     • red
                                       • sucrose or fructose
                     • blue
                       • starch
                         • fehling test
                           • FA+ FB + 2 ml carbohydrate + heat
                             • no red
                               • sucrose or starch

2. Biuret test

   • 3 ml protein + 1 drop CuSo4 + 1 ml NaOH
     • violet color
       • protein
         • heat coagulation tes
           • 15 ml protein + heat
             • white coagulation = egg white
               • reaction test
                 • alkaline = casiene
                   • rosenheim test
                     • 3 ml protein + 2-3 drops ferric chloride formaldhide + H2SO4
                       • violet ring
                         • eggwhite or casiene
                       • no violet ring
                         • gelatin
                 • neutral= eggwhite or gelatin
                   • rosenheim test
                     • 3 ml protein + 2-3 drops ferric chloride formaldhide + h2so4
                       • no violet ring = geltin
                       • violet ring = eggwhite or casiene