DNA (labeling) for biochem and cell biology (lecture 2)

Description

1st year Biochemistry and molecular biology Quiz on DNA (labeling) for biochem and cell biology (lecture 2), created by MrSujg on 11/29/2015.
MrSujg
Quiz by MrSujg, updated more than 1 year ago
MrSujg
Created by MrSujg about 7 years ago
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11

Resource summary

Question 1

Question
Label the image
Answer
  • 1'
  • 2'
  • 3'
  • 4'
  • 5'
  • alpha
  • Beta
  • gamma
  • base

Question 2

Question
What base is it ?
Answer
  • Adenine
  • Guanine
  • Cytosine
  • Thymine
  • Uracil

Question 3

Question
What base is it ?
Answer
  • Guanine
  • Adenine
  • Cytosine
  • Thymine
  • Uracil

Question 4

Question
What base is it?
Answer
  • Cytosine
  • Adenine
  • Guanine
  • Thymine
  • Uracil

Question 5

Question
What base is it?
Answer
  • Thymine
  • Adenine
  • Uracil
  • Cytosine
  • Guanine

Question 6

Question
What base is it?
Answer
  • Uracil
  • Adenine
  • Guanine
  • Tymine
  • Cytosine

Question 7

Question
Identify primers
Answer
  • 3'
  • 5'

Question 8

Question
Label the image
Answer
  • 5'
  • 3'
  • 5'
  • 3'
  • alpha
  • beta
  • gamma
  • O
  • C
  • S
  • H
  • N
  • F
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III

Question 9

Question
Label the image
Answer
  • Minor groove
  • Major groove

Question 10

Question
Label the image
Answer
  • Guanine
  • Cytosine
  • Thymine
  • Adenine

Question 11

Question
Determine the primers
Answer
  • 5'
  • 3'
  • 3'
  • 5'
  • 3'
  • 5'
  • 5'
  • 3'

Question 12

Question
Label the image
Answer
  • A-DNA
  • B-DNA
  • Z-DNA

Question 13

Question
Label the image
Answer
  • Conservative mechanisms
  • Semiconservative mechanism

Question 14

Question
Label the image
Answer
  • Origin
  • Growing fork

Question 15

Question
Label the image
Answer
  • EcorRI restriction site
  • Origin
  • Replication bubble
  • Time of replication

Question 16

Question
Labelt the replication cartoon
Answer
  • Primase
  • SIngle0stranded region of parent DNA
  • RNA primer
  • DNA polymerase III
  • Newly made DNA
  • DNA polymerase I
  • RNA still to be replaced
  • Newest DNA
  • Completed daughter cell

Question 17

Question
Label the cartoon showing the unwinding at the origin
Answer
  • 9-mers
  • Negatively supercoiled template
  • DnaA
  • ATP
  • Initial complex
  • Open complex
  • DnaC
  • DnaB (helicase)
  • Prepriming complex

Question 18

Question
Label the cartoon showing Simultaneous synthesis on both strands
Answer
  • Point of joining
  • Lagging strand
  • Okazaki fragment
  • Short RNA primer
  • Leading strand
  • Parental DNA duplex
  • Daughter duplex
  • Direction of fork movement

Question 19

Question
Label the cartoon showing the participitation of topoisomers in DNA unwinding
Answer
  • Covalently closed circular template
  • DNA replication
  • Unwound parental duplex
  • Negative supercoils
  • Positive supercoils
  • Overwound region

Question 20

Question
Label DNA polymerase
Answer
  • Fingers
  • Thumb
  • Growing strand
  • Template strand
  • Exo
  • Palm
  • Pol

Question 21

Question
Label the image showing mismatch repair
Answer
  • Daughter strand
  • Parental strand
  • Mismatched base pair
  • Mismatch recognition
  • MutS
  • MutH
  • MutH cleavage
  • Gap repair by DNA polymerase and ligase

Question 22

Question
Label the cartoon showing excision repair
Answer
  • Initial damage recognition
  • 23B
  • XP-C
  • Opening of DNA double helix
  • TFIIH
  • XP-G
  • RPA
  • XP-F and XP-G endonucleases
  • DNA polymerase and DNA ligase

Question 23

Question
Label the image showing PCR
Answer
  • DNA Denaturation, annealing of primers
  • Elongation of primers
  • DNA denaturation; annealing or primers 2
  • Elongation of primers 2

Question 24

Question
Label the image
Answer
  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase 3

Question 25

Question
Label the image showing the Transcription in Prokaryotes
Answer
  • Promoter DNA
  • -35 sequence
  • 10 sequence
  • Coding strand
  • Template strand
  • Startpoint

Question 26

Question
Labelt the image showing A-Binding Domains (DBDs)
Answer
  • Helix-turn-helix motif
  • The lambda repressor
  • Zinc finger motif
  • Leucine zipper motif
  • Helix-loop-helix motif

Question 27

Question
Label the image showing the lac operon activation
Answer
  • Beta-galactosidase
  • RNA polymerase bound to promoter
  • lac-mRNA
  • Lactose
  • RNA polymerase transcribing the operon
  • lacI gene
  • Inactive form of repressor

Question 28

Question
Label the image showing the regulation of the lac Operon
Answer
  • Promoter
  • CAP site
  • CAP
  • a70-Pol
  • lac repressor
  • Lactose
  • cAMP

Question 29

Question
Label the image showing Eukaryotic promoters
Answer
  • GC box
  • CAAT box
  • TATA box
  • Proximal control elements
  • Core promoter
  • Start of transcription
  • Poly (A) site
  • 5' untranslated region (leader)
  • 3' untranslated region (trailer)

Question 30

Question
label the image showing General Eukaryotic Transcription Factors
Answer
  • Core promoter
  • Startpoint
  • TFIID
  • TFIIA
  • TFIIB
  • TFIIF
  • RNA polymerase 2
  • TFIIE
  • TFIIH
  • Transcription begins

Question 31

Question
Label the image showing Additional Eukaryotic Transcription Factors
Answer
  • Enhancer
  • Core promotor
  • TATA
  • Inr
  • Gene
  • Activators
  • Coactivators
  • Enhanceosome
  • TFIID
  • Activator and Coactivator
  • Transcription

Question 32

Question
label the image showing Tissue-Specific Gene Expression
Answer
  • Control elements
  • Core promoter
  • Regulatory transcription factors
  • General transcription factors
  • RNA polymerase
  • Transcription complex
  • DNA
  • RNA transcript

Question 33

Question
Label the image showing how the final mRNA is made
Answer
  • Exon
  • Intron
  • Poly (A) site
  • Termination sites
  • Transcription 5' capping
  • Cleavage at poly (A) site
  • Polyadenylation
  • RNA splicing

Question 34

Question
Labe the image showing Microarrays: Genome-Wide Analysis
Answer
  • RNA isolation
  • Reverse transcriptase labeling
  • Combine targets
  • Hybridaze to microarray

Question 35

Question
Label the image showing the molecular Dogma
Answer
  • Transcription
  • Translation
  • Assembly
  • tRNA
  • rRNA
  • Ribosomes
  • Polypeptides
  • Proteins
  • Ribosomal proteins

Question 36

Question
Label the image
Answer
  • Primary RNA transcript
  • 5'
  • 3'
  • DNA
  • Promoter
  • Transcription
  • Coding sequence
  • Upstream
  • Downstream

Question 37

Question
Follow the genetic code to match the appropriate amino acid to each codon
Answer
  • Ala
  • Cys
  • Leu
  • Arg
  • Ile
  • Val
  • Tyr
  • Glu

Question 38

Question
Label the type of the mutation
Answer
  • Frameshift
  • Missense
  • Nonsense

Question 39

Question
Identify the type of ribosome, and the size of the assembled unit
Answer
  • Prokaryotic
  • Eukaryotic (vertebrate)
  • 70S
  • 80S

Question 40

Question
Label the image showing aminoacyl-tRNAsynthetases
Answer
  • High-energy ester bond
  • Amino acid (Phe)
  • mRNA
  • Aminoacyl-tRNA
  • tRNA specific for Phe

Question 41

Question
Label the image
Answer
  • Adenosine deaminase
  • Adenosine
  • Inosine

Question 42

Question
Label the initiator methionine
Answer
  • Formyl group
  • Carboxyl group
  • Acetyl group
  • Phosphate group

Question 43

Question
Label each mRNA
Answer
  • Prokaryotic mRNA
  • Eukaryotic mRNA

Question 44

Question
Label the ribosome sites
Answer
  • A site
  • P site
  • E-site
  • mRNA-binding site
  • mRNA
  • Growing peptide chain

Question 45

Question
Label the image showing translation
Answer
  • P site
  • E site
  • A site
  • Release factor
  • free tRNA
  • Polypeptide
  • Ribosomal subunits
  • mRNA

Question 46

Question
Label the image showing translation mechanism
Answer
  • AUG start codon
  • UAG stop codon
  • mRNA
  • Initiation
  • Elongation
  • Termination
  • Release factor
  • Completed polypeptide
  • Recycling of translational components
  • Ribosomal subunits

Question 47

Question
Label the image showing western blotting
Answer
  • Electrophoresis and transfer
  • Antibody detection
  • Chromogenic detection
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