DNA Replication & Mitosis

DNA Replication & Mitosis

1. Cell Cycle

   • Prokaryote
     • Binary Fission
   • Eukaryote
     • Stem Cells
       • Quiescent Satellite Cell
     • Interphase
       • Mitosis
         • Cytokinesis
           • Contractile Ring
           • Cell Plate Region
       • S phase
       • G1
       • G2
   • Chromosome dynamics
     • Kinetochores
     • Polar microtubules
   • Controlling progression
     • Kinase
       • P53 Gene
         • Cyclin-CDK
           • Checkpoints
     • Checkpoints
       • DNA Damage
         • DNA Replication
           • Spindle Assembly

2. Replication

   • Semi-conservative
     • Meselson & Stahl
   • Prokaryote
     • 1 origin of rep.
   • Complex
     • Topoisomerase II
       • RELIEVES STRESS
       • Helicase
         • UNWINDS
         • Single Stranded Binding Protein
           • STABILIZES & PREVENTS REJOINING
           • RNA Primase
             • Initiation - attach RNA primer
             • DNA Polymerase III
               • Elongation
               • DNA Polymerase I
                 • Removes RNA primer
               • Proofread errors
   • Eukaryote
     • Continuous
       • Leading Strand
     • Discontinuous
       • Lagging Strand
         • Okazaki fragments
           • DNA Ligase
     • Telomeres
       • Telomerase

3. Applications of DNA Replication

   • PCR
     • Denaturation
     • Annealing
     • Extension
   • Gel Electrophoresis
   • DNA Sequencing
     • Shotgun
       • "Contigs"
     • Sanger
       • Chain terminators
         • Addition of ddNTP
     • Annotation
       • "sequence motifs"

4. DNA Mutations

   • Somatic
   • Germline
   • Replica Plating
   • DNA Repair
     • Mismatch
     • Base Exicison
     • Nucleotide Excision
   • Nucleotide Seq.
     • Point Mutation
       • Synomous
       • Nonmutant
       • Nonsynonmous
       • Nonsense
   • Chromosomal
     • Deletion
     • Duplication
     • Inversion
     • Reciprocal Translocation
   • Gene Evolution
     • gamma & beta globin