determine the effect of calcium ions on the coagulation of milk

determine the effect of calcium ions on the coagulation of milk

1. The reaction is catalysed by the enzyme rennin

   • Enzymes are protein molecules that act as biological catalysts
     • Look at what effects enzyme activity short notes
     • what effects enzyme activity
   • Calcium ions are cofactors for the enzyme rennin by binding to either the substrate or enzyme they make the formation of enzyme-substrate complex easier.(affects charge distribution and the shape of the enzyme-substrate complex)
     • So lower concentration of calcium ions will decrease the rate of the reaction.
     • While higher concentration of calcium ions will increase the rate of the reaction

2. Sodium substrate has to be added as to remove all the calcium ions that are normally present in milk

   • this than allows as to test the effect of different concentrations of calcium ions.