Mass Spectrometry

The Mass Spectrometer

• Mass spectrometers can be used to determine all the isotopes present in a sample of an element and to therefore identify elements.
• Calculating relative atomic mass
• The relative atomic mass quoted on the periodic table is a weighted average of all the isotopes of the element.
• For each isotope the mass spectrometer can measure a m/z (mass/charge ration) and an abundance.
• If asked to give a species from a mass spectrum then give charge and mass number.
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• Legenda: Spectra for magnesium from mass spectrometer

Slide 2

• Sometimes two electrons may be removed from a particle forming a 2+ ion. ^24Mg^2+ with a 2+ charge would have an m/z of 12 (24/2).
• Use this equations to work out RAM:
• RAM = isotopic mass x %abundance/100
• For above example - RAM = [(78.7 x 24) + (100.13 x 25) + (11.17 x 26)]/100 = 24.3
• Or if relative abundance is used instead of percentage abundance:
• RAM = isotopic mass x relative abundance/total relative abundance

Mass Spectra for Cl2 and Br2

• Cl has two isotopes: Cl^35 (75%) and Cl^37 (25%).
• Br has two isotopes: Br^79 (50%) and Br^81 (50%).
• These lead to the following spectra caused by the diatomic molecules -->

Slide 4

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• Legenda: The first two lines in each spectra show the masses of the two isotopes.
• Legenda: The other lines are the combinations of these values added together: e.g. 79 + 79 = 158 The different column heights show the ratio of each combination.

Measuring the Mr of a molecule

• If a molecule is put through a mass spectrometer it will often break up and give a series of peaks caused by the fragments. The peak giving the largest m/z, however, will be due to the complete molecule and will be equal to the Mr of the molecule. This peak is called the parent or molecular ion.
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Uses of mass spectrometers

• Mass spectrometers have been used:
• in planetary space probes so tat elements on other planets can be identified (they can have different compositions of isotopes)
• for drug testing in sports to identify chemicals in the blood and breakdown products from drugs in the body
• for quality control in the pharmaceutical industry and to identify molecules from sample with potential biological activity
• radioactive dating to determine the age of fossils and human remains