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Quiz

Enterobacteriaceae

Katie Koontz 7 perguntas 1.459 visualizações Atualizado em 14 set 2016

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  1. 1Purpose: TSI agar can be considered an initial step in the identification of the [blank_start]Enterobacteriaceae[blank_end]
    1. AEnterobacteriaceae
    2. BSalmonella typhi
    3. CEscherichia coli
    4. DCitrobacter diversus
  2. 2Principle: for [blank_start]Indole Broth[blank_end]: tryptophan PRESENT in peptone is oxidized y certain bacteria to indole, skatole, and indoleacetic acid. Indole is detected in broth cultures of bacteria with an alcoholic p-dimethylaminobenzldehyde reagent. Indole reacts with the aldehyde to form a [blank_start]red[blank_end] product.
    1. AIndole Broth
    2. BUrea Agar
    3. CVoges-proskauer
    4. DMethyl red
    5. Ered
    6. Fblack
    7. Gyellow
    8. Hgray
  3. 3Key Ingredients: [blank_start]Indole broth[blank_end]: Tryptophan (1% broth), Kovac's reagent or Ehrlich's reagent, Xylene or chloroform for extraction if using Ehrlich's reagent
    1. AIndole broth
    2. BMethyl Red
    3. CPhenylalanine
    4. DLIA
  4. 4Indole Broth Reactions:
    Negative Reaction: [blank_start]No[blank_end] color development
    Positive Reaction: [blank_start]red[blank_end] ring at the interfacec of reagent and broth)
    Variable reaction: [blank_start]Orange[blank_end] color, indicates production of aktole, a methylated intermediate that may be a precursor to indole production
    1. ANo
    2. BRed
    3. CDark
    4. DYellow
    5. Ered
    6. Fyellow
    7. Gno
    8. Hbrown
    9. IOrange
    10. JRed
    11. KBlack
    12. LPurple
  5. 5Principle: Urea Hydrolysis (Christensen's Agar): Many organisms possess the enzyme urease which can hydrolyze urea with the release of ammonia and carbon dioxide which acts to increase the pH of the medium and turn the indicator phenol red from pale [blank_start]orange[blank_end] to dark [blank_start]fuchsia pink[blank_end]
    1. Aorange
    2. Bfuchsia pink
    3. Cviolet purple
    4. Dgreen
    5. Efuchsia pink
    6. Forange
    7. Gviolet purple
    8. Hgreen
  6. 6Key Ingredients: Urea Agar
    Urea Agar ([blank_start]christensen's Base[blank_end])
    pH indicator = [blank_start]phenol red[blank_end]
    1. Achristensen's Base
    2. Bmethyl red
    3. Cspot indole broth
    4. Dcitrate
    5. Ephenol red
    6. Fmethyl red
    7. Ggelatin
    8. HAPI 20E strip
  7. 7Interpretation: urea hydrolysis
    A strong positive hydrolysis test: entire agar, slant and butt, turning [blank_start]deep pink[blank_end] after overnight incubation.
    A moderate positive test: [blank_start]pink[blank_end] slant at 24 hours, with the intensity of the color increasing with extended incubation
    A negative test: no change from pale [blank_start]orange[blank_end] color of the slant
    1. Adeep pink
    2. Bdeep purple
    3. Clight orange
    4. Dlight pink
    5. Epink
    6. Forange
    7. Gyellow
    8. Hred
    9. Iorange
    10. Jpink

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Descrição
All about enterobacteriaceae
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English
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1.459
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Criado em 12 set 2016 · atualizado em 14 set 2016
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