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Microbiology Practical 2
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1Eosin-methylene blue plates select for Gram [blank_start]negative[blank_end] [blank_start]enteric[blank_end] bacteria, and differentiates [blank_start]lactose[blank_end] fermentation.
- Anegative
- Benteric
- Clactose
2EMB plates selective agents are [blank_start]eosin[blank_end] and [blank_start]methylene[blank_end] [blank_start]blue[blank_end]. The differential reagent is [blank_start]methylene[blank_end] [blank_start]blue[blank_end].
- Aeosin
- Bmethylene
- Cblue
- Dmethylene
- Eblue
3On EMB plates, E. coli will have a [blank_start]green[blank_end] sheen, Enterbacter genus will be [blank_start]pink[blank_end] with [blank_start]black[blank_end] edges. Lactose fermenting bacteria will be [blank_start]pink[blank_end] and non-lactose fermenting will be [blank_start]cream[blank_end].
- Agreen
- Bpink
- Cblack
- Dpink
- Ecream
4Phenyl ethyl Alcohol plates select for Gram [blank_start]positive[blank_end] bacteria especially the shape [blank_start]cocci[blank_end].
- Apositive
- Bcocci
5The selective agent of PEA is [blank_start]phenyl[blank_end] [blank_start]ethyl[blank_end] [blank_start]alcohol[blank_end].
- Aphenyl
- Bethyl
- Calcohol
6In the selective and differential media plates, the only plate that was ONLY selective and not differential was the [blank_start]phenyl[blank_end] [blank_start]ethyl[blank_end] [blank_start]alcohol[blank_end] plate.
- Aphenyl
- Bethyl
- Calcohol
7MacConkey's plate selects for Gram [blank_start]negative[blank_end] bacteria and differentiates for [blank_start]lactose[blank_end] fermentation with positive being [blank_start]pink[blank_end] to [blank_start]purple[blank_end] and negative being [blank_start]cream[blank_end].
- Anegative
- Blactose
- Cpink
- Dpurple
- Ecream
8The MAC plate selective agent is the [blank_start]bile[blank_end] [blank_start]salts[blank_end] and the differential reagent is [blank_start]neutral[blank_end] [blank_start]red[blank_end].
- Abile
- Bsalts
- Cneutral
- Dred
9The Mannitol Salt agar selects for [blank_start]salt[blank_end] tolerance and especially the Genus [blank_start]Staphylococci[blank_end]. A Positive result will have a [blank_start]yellow[blank_end] halo around colony, and a negative result will have no halo.
- Asalt
- BStaphylococci
- Cyellow
10The selective agent for MSA plate is [blank_start]7.5[blank_end]% [blank_start]NaCl[blank_end] and the differential reagent is [blank_start]phenol[blank_end] [blank_start]red[blank_end].
- A7.5
- BNaCl
- Cphenol
- Dred
11[blank_start]Tryptic[blank_end] [blank_start]Soy[blank_end] [blank_start]Agar[blank_end] or [blank_start]TSA[blank_end] plate allows for widest amount of growth and is often the control plate when using selective and differential media.
- ATryptic
- BSoy
- CAgar
- DTSA
12EMB plates inhibit Gram [blank_start]positive[blank_end] organisms and shows lactose fermenters but also [blank_start]sucrose[blank_end]. The color also indicates speed of fermentation. [blank_start]Green[blank_end] is rapid and [blank_start]purple[blank_end] is moderate.
- Apositive
- Bsucrose
- CGreen
- Dpurple
13Escherichia coli will be present on what plates?
- AEosin-methylene blue
- BPhenyl ethyl Alcohol
- CMacConkeys
- DMannitol Salt Agar
14Escherichia coli is a [blank_start]bacillus[blank_end] (shape) Gram [blank_start]negative[blank_end] bacteria which is [blank_start]falcultative[blank_end] [blank_start]anaerobic[blank_end] (oxygen requirements) and is a [blank_start]coliform[blank_end] (where it lives) meaning it is an [blank_start]enteric[blank_end] bacteria.
- Abacillus
- Bnegative
- Cfalcultative
- Danaerobic
- Ecoliform
- Fenteric
15[blank_start]Escherichia[blank_end] [blank_start]coli[blank_end] is present on an EMB plate as a green sheen and on a MAC plate as pink/purple colonies.
- AEscherichia
- Bcoli
16Enterobacter aerogenes is present on what plates?
- AEosin-methylene blue
- BPhenyl ethyl Alcohol
- CMacConkeys
- DMannitol Salt Agar
17[blank_start]Enterobacter[blank_end] [blank_start]aerogenes[blank_end] is present on EMB plate as pink with black edges and pink/purple on MAC.
- AEnterobacter
- Baerogenes
18Enterobacter aerogenes is a Gram [blank_start]negative[blank_end] bacteria that ferments [blank_start]lactose[blank_end] and is [blank_start]enteric[blank_end] (location) and is [blank_start]bacillus[blank_end] (shape).
- Anegative
- Blactose
- Centeric
- Dbacillus
19Enterobacter aerogenes and Escherichia coli will look the same on a MacConkeys plate. The way to differentiate them using plates is through the [blank_start]eosin[blank_end] [blank_start]methylene[blank_end] [blank_start]blue[blank_end] plate.
- Aeosin
- Bmethylene
- Cblue
20Staphylococcus aureus will be present on what plates?
- AEMB
- BPEA
- CMAC
- DMSA
21Staphylococcus aureus is a Gram [blank_start]positive[blank_end] [blank_start]cocci[blank_end] (shape) bacteria that is [blank_start]salt[blank_end] tolerant and can ferment [blank_start]mannitol[blank_end].
- Apositive
- Bcocci
- Csalt
- Dmannitol
22[blank_start]Staphylococcus[blank_end] [blank_start]aureus[blank_end] would show growth on a Phenyl ethyl alcohol plate and grow yellow colonies on an MSA plate.
- AStaphylococcus
- Baureus
23Serratia marcescens is a Gram [blank_start]negative[blank_end] [blank_start]bacillus[blank_end] (shape) bacteria is not tolerant of [blank_start]salt[blank_end] and does not ferment [blank_start]lactose[blank_end].
- Anegative
- Bbacillus
- Csalt
- Dlactose
24Serratia marcescens will be present on what plates?
- AEMB
- BPEA
- CMAC
- DMSA
25Bacillus cereus is present on what plates?
- AEMB
- BPEA
- CMAC
- DMSA
26The [blank_start]Catalase[blank_end] test indicates the presence of the enzyme [blank_start]catalase[blank_end] which turns hydrogen peroxide into water and oxygen. A positive result is indicated by bubbles.
- ACatalase
- Bcatalase
27The DNase test is completed on a plate that is blue-green from having a [blank_start]methyl[blank_end] [blank_start]green[blank_end] dye and [blank_start]DNA[blank_end].
- Amethyl
- Bgreen
- CDNA
28A positive result on the [blank_start]DNase[blank_end] test is indicated by a [blank_start]clearing[blank_end] around the bacteria smear as hydrolysis of the DNA releases it form the methyl green dye.
- ADNase
- Bclearing
29The Urease test indicates the presence of urease. The media contains the pH indicator [blank_start]phenol[blank_end] [blank_start]red[blank_end] so a positive test is indicated by the color [blank_start]pink[blank_end].
- Aphenol
- Bred
- Cpink
30The Urease test uses the following chemical reaction to indicate positive or negative.
[blank_start]Urea[blank_end] + [blank_start]Water[blank_end] -> [blank_start]carbon dioxide[blank_end] + [blank_start]water[blank_end] + [blank_start]Ammonia[blank_end]
The last product is basic so the pH indicator in the media indicates presence of the product and the forward reaction.- AUrea
- BWater
- Ccarbon dioxide
- Dwater
- EAmmonia
31[blank_start]Casein[blank_end] [blank_start]Hydrolysis[blank_end] is completed on a milk plate to determine if microbes can break down the milk protein [blank_start]casein[blank_end]. A clearing around the bacteria smear indicates a [blank_start]positive[blank_end] result.
- ACasein
- BHydrolysis
- Ccasein
- Dpositive
32During Casein hydrolysis, casein is broken down to [blank_start]amino[blank_end] [blank_start]acids[blank_end] and [blank_start]peptides[blank_end]
- Aamino
- Bacids
- Cpeptides
33Starch hydrolysis tests for the enzymes [blank_start]amylase[blank_end] and [blank_start]maltase[blank_end]. [blank_start]Amylase[blank_end] is used when breaking down starch to [blank_start]dextrins[blank_end], and [blank_start]dextrins[blank_end] to [blank_start]maltose[blank_end]. [blank_start]Maltase[blank_end] is used to breakdown [blank_start]maltose[blank_end] to glucose.
- Aamylase
- Bmaltase
- CAmylase
- Ddextrins
- Edextrins
- Fmaltose
- GMaltase
- Hmaltose
34After the bacteria is cultured on the starch plate, [blank_start]iodine[blank_end] is added. A blue-black color indicates starch is still intact (meaning it is [blank_start]negative[blank_end]), whereas a [blank_start]clear[blank_end] area indicates starch hydrolysis.
- Aiodine
- Bnegative
- Cclear
35The oxidase test detects the presence of the enzyme [blank_start]cytochrome[blank_end] [blank_start]oxidase[blank_end]. This is an electron carrier protein involved in the electron transport system.
- Acytochrome
- Boxidase
36In the oxidase test, a positive result has the oxidase reagent, [blank_start]phenylenediamine[blank_end], oxidize and turns purple.
- Aphenylenediamine
37The fermentation tubes used the indicator [blank_start]phenol[blank_end] [blank_start]red[blank_end] to detect pH changes. If the microbe fermented the sugar, the color would turn [blank_start]yellow[blank_end]. [blank_start]Durham[blank_end] tubes indicate the production of gases either carbon dioxide or hydrogen.
- Aphenol
- Bred
- Cyellow
- DDurham
38the [blank_start]triple[blank_end] [blank_start]sugar[blank_end] [blank_start]iron[blank_end] is a mutli-faceted test that looks at the fermentation of glucose, sucrose, or lactose and can indicate H2S production.
- Atriple
- Bsugar
- Ciron
39In the triple sugar iron test, the positive color is [blank_start]yellow[blank_end], the negative color is [blank_start]pink[blank_end], and the color [blank_start]black[blank_end] in the butt indicates H2S production
- Ayellow
- Bpink
- Cblack
40In the triple sugar iron test, a positive slant indicates [blank_start]sucrose[blank_end] or [blank_start]lactose[blank_end] fermentation, a yellow butt indicates [blank_start]glucose[blank_end] fermentation, a black butt indicates [blank_start]glucose[blank_end] fermentation and [blank_start]H2S[blank_end] production. Space in the butt indicates [blank_start]gas[blank_end] production.
- Asucrose
- Blactose
- Cglucose
- Dglucose
- EH2S
- Fgas
41The IMViC test series includes the following tests:
1. [blank_start]Indole[blank_end] test
2. [blank_start]MRVP[blank_end] (abbr.) [blank_start]Methyl[blank_end] [blank_start]Red[blank_end] [blank_start]Vogues[blank_end]-[blank_start]Proskauer[blank_end]
3. [blank_start]Citrate[blank_end]- AIndole
- BMRVP
- CMethyl
- DRed
- EVogues
- FProskauer
- GCitrate
42The indole test detects the product Indole which is created by the enzyme [blank_start]Tryptophanase[blank_end] which breaks down [blank_start]tryptophan[blank_end] to [blank_start]pyruvate[blank_end], [blank_start]NH3[blank_end] and [blank_start]indole[blank_end].
- ATryptophanase
- Btryptophan
- Cpyruvate
- DNH3
- Eindole
43In the Indole test, the reagent is [blank_start]Kovac[blank_end]'s reagent. A [blank_start]MRVP[blank_end] broth culture is used as the parent which is then divided to different tubes for the different tests. A positive indole test will have a [blank_start]red[blank_end] band at the top of the solution. Negative will be [blank_start]yellow[blank_end].
- AKovac
- BMRVP
- Cred
- Dyellow
44Methyl Red detects various [blank_start]acid[blank_end] production. A [blank_start]red[blank_end] color indicates it is [blank_start]acidic[blank_end] meaning a positive test, [blank_start]yellow[blank_end] is neutral or negative test.
- Aacid
- Bred
- Cyellow
- Dacidic
45The Vogues-Proskauer test detects the production of [blank_start]acetoin[blank_end] which is the precursor to the product [blank_start]2,3-butanediol[blank_end].
- Aacetoin
- B2,3-butanediol
46The VP test uses the reagents [blank_start]alpha[blank_end]-[blank_start]naphthol[blank_end] and [blank_start]potassium[blank_end] [blank_start]hydroxide[blank_end]. A positive test has a [blank_start]red[blank_end] color near the top of the tube.
- Aalpha
- Bnaphthol
- Cpotassium
- Dhydroxide
- Ered
47The VP test must be mixed every [blank_start]15[blank_end] seconds for 2 minutes, and then let to sit for 15 minutes.
- A15
48The Citrate Agar slant tests for the microbes ability to use citrate as its sole [blank_start]carbon[blank_end] source. A microbe must have the enzyme [blank_start]citrate[blank_end] [blank_start]permease[blank_end] which is a transport protein which produces an [blank_start]alkaline[blank_end] pH.
- Acarbon
- Bcitrate
- Cpermease
- Dalkaline
49The pH indicator used on the Citrate agar slant is [blank_start]Bromothymol[blank_end] [blank_start]blue[blank_end]. A positive test is [blank_start]blue[blank_end], a negative is [blank_start]green[blank_end].
- ABromothymol
- Bblue
- Cblue
- Dgreen
50The fermentation tubes tested for [blank_start]glucose[blank_end], [blank_start]lactose[blank_end], [blank_start]mannose[blank_end], and [blank_start]sucrose[blank_end] fermentation.
- Asucrose
- Bmannose
- Clactose
- Dglucose
51MSA plates test for [blank_start]halo[blank_end]-tolerant species and [blank_start]mannitol[blank_end] sugar fermentation. Acid fermentation will have a [blank_start]yellow[blank_end] color and alkaline will be [blank_start]pink[blank_end].
- Ahalo
- Bmannitol
- Cyellow
- Dpink
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- Microbiology Quiz on Microbiology Practical 2, created by Rachel Rivera on 23/11/2018.
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- Rachel Rivera
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