16253851
Description
Quiz by Olivia Muench, updated more than 1 year ago
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Created by Olivia Muench
over 5 years ago
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|
Question 1
Question
Microbiology Lab is Biosafety Level 2
Answer
- True
- False
Question 2
Question
Bacteria are [blank_start]ubiquitous[blank_end], since they can be found all over and in every environment.
Answer
-
ubiquitous
-
formite
-
thick
Question 3
Question
Bacteria have a [blank_start]negative charge[blank_end]. Because of this basic dyes, with their [blank_start]positive charge[blank_end], stain the [blank_start]bacteria[blank_end]. Acidic dyes, with their [blank_start]negative charge[blank_end], stain the [blank_start]background[blank_end].
Answer
-
negative charge
-
positive charge
-
positive charge
-
negative charge
-
negative charge
-
positive charge
-
bacteria
-
background
-
background
-
bacteria
Question 4
Question
Examples of acidic dyes:
Answer
-
Eosin
-
Methylene Blue
-
Crystal Violet
-
Nigrosin
-
Safranin
-
Congo Red
-
Maneval's stain
Question 5
Question
Which microscope was used in the lab?
Answer
-
Dark-field microscope
-
Phase contrast microscope
-
Bright-field microscope
-
Flourescence microscope
Question 6
Question
Limit of Resolution:
D = [blank_start]Wavelength[blank_end] / ([blank_start]NA condensor[blank_end] + [blank_start]NA objective lens[blank_end])
Answer
-
Wavelength
-
NA condensor
-
NA objective lens
-
Wavelength
-
NA condensor
-
NA objective lens
-
Wavelength
-
NA condensor
-
NA objective lens
Question 7
Question
Smears should:
Answer
-
withstand washing
-
cover the entire slide
-
not be too thick
-
not result in excessive distortion of the organism
-
fall off the slide
Question 8
Question
Purpose of heat-fixing
Answer
-
kill bacteria
-
kill you
-
fix the bacteria to the slide
-
make cells more visible by coagulating cytoplasmic proteins
Question 9
Question
Pure cultures should have many different kinds of bacteria
Answer
- True
- False
Question 10
Question
Klebsiella Pneumoniae has a capsule
Answer
- True
- False
Question 11
Question
Choose what best describes capsules
Answer
-
Made of a polysaccharide layer called glycocalyx
-
Water soluble
-
Fat soluble
-
Heat sensitive
-
Prevents dehydration/dessication
-
Decreases virulence by protecting the cell from phagocytosis
-
Helps attachment to host cell
Question 12
Question
Gram-positive bacteria
Answer
-
Have an outer membrane/lipopolysaccharide layer (LPS)
-
Cell wall is ~90% peptidoglycan and has teichoic acid
-
Cell wall is ~10% peptidoglycan
-
are stained pink/red with safranin
Question 13
Question
Order of Gram staining:
1. [blank_start]Primary stain[blank_end] with [blank_start]Crystal Violet[blank_end]
2. [blank_start]Mordant[blank_end] with [blank_start]Gram's Iodine[blank_end]
3. [blank_start]Decolorizer[blank_end] with [blank_start]95% Ethanol[blank_end]
4. [blank_start]Counterstain[blank_end] with [blank_start]Safranin[blank_end]
Answer
-
Primary stain
-
Crystal Violet
-
Mordant
-
Gram's Iodine
-
Decolorizer
-
95% Ethanol
-
Counterstain
-
Safranin
Question 14
Question
Which describes chemotaxis?
Answer
-
organism vibrates due to molecules hitting the cell
-
directional; independent movement over greater distances
-
movement towards chemical attractants and away from repellants
Question 15
Question
Which describes lophotrichous flagella?
Answer
-
flagella on both ends
-
flagella around perimeter of bacteria
-
a single flagella at one end
-
a tuft of flagella at one end
Question 16
Question
The motility test media is 0.4-0.5% agar and contains TTC (2,3,5-triphenyltetrazolium chloride)
Answer
- True
- False
Question 17
Question
Disadvantages of the motility test:
Answer
-
can be used to test for motility of pathogenic organisms
-
requires incubation time
-
if not performed properly, non-motile organisms appear motile
Question 18
Question
TTC is colorless when [blank_start]oxidized[blank_end] and red when [blank_start]reduced[blank_end].
Answer
-
oxidized
-
reduced
Question 19
Question
Escherichia coli is motile and Staphylococcus epidermidis is non-motile.
Answer
- True
- False
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