Microbiology quiz 1 ( Part 2 )

Laura Pepe
Quiz by Laura Pepe, updated more than 1 year ago
Laura Pepe
Created by Laura Pepe about 1 year ago
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Univeristy Microbiology Quiz on Microbiology quiz 1 ( Part 2 ), created by Laura Pepe on 01/30/2020.

Resource summary

Question 1

Question
How do we study microbes and all the jazz?
Answer
  • Culture them
  • Ingest them
  • Look at them under a microscope
  • Study their DNA, RNA, and proteins!

Question 2

Question
Complex media is harder to make and every single ingredient is measured.
Answer
  • True
  • False

Question 3

Question
General Media has which of the following characteristics?
Answer
  • Super selective
  • Great Plate Count Anomaly
  • Still can’t culture everything
  • Cultures as many microbes as possible

Question 4

Question
Enrichment media has which of the following characteristics/example(s)?
Answer
  • Culture fastidious organisms
  • Chocolate agar
  • Mannitol Agar
  • Add things to the media to enable specific types of bacteria to grow

Question 5

Question
[blank_start]Selective[blank_end] media selects for certain bacteria and selects against other bacteria.
Answer
  • Selective
  • Enriched
  • Differential
  • General

Question 6

Question
Differential media usually is dependent on bacterial metabolic activity.
Answer
  • True
  • False

Question 7

Question
Differential media is not similar or at all like selective media.
Answer
  • True
  • False

Question 8

Question
Choose the correct terms for the following characteristics of autoclaving. Autoclaving uses high [blank_start]pressure[blank_end] and high temperature to kill even [blank_start]endospores[blank_end]. [blank_start]Wet[blank_end] heat has more energy than dry heat. This kills everything but can be [blank_start]bad[blank_end] for delicate or reactive compounds. Autoclaving can also cause [blank_start]vitamins[blank_end] to [blank_start]denature[blank_end]. Also, trace metals that are [blank_start]redox[blank_end]-sensitive might react and become unavailable.
Answer
  • pressure
  • endospores
  • Wet
  • bad
  • vitamins
  • denature
  • redox

Question 9

Question
If autoclaving can denture vitamins and ruin or cause trace metal react, then what is another method of sterilization that would be better suited to handle this problem?
Answer
  • UV light
  • Filter sterilization
  • Bleach
  • Clorox Bleach-Free Fabric Sanitizer

Question 10

Question
Which of the following is TRUE about filtered sterilization?
Answer
  • Used for reactive compounds (vitamins or trace metals)
  • Removes the cells from the liquid, as autoclaving kills cells but doesn’t necessarily destroy them.
  • Requires UV lamp
  • Need cell-free water to do dilutions for microscope counts

Question 11

Question
Which is not a form of UV light sterilization?
Answer
  • UV-2
  • UV-A
  • UV-C
  • UV-B

Question 12

Question
Order the strength of UV light sterilization in increasing order in terms of effectiveness of killing microbes: 1) Lowest --> [blank_start]UV-A[blank_end] 2) Medium --> [blank_start]UV-B[blank_end] 3) Highest --> [blank_start]UV-C[blank_end]
Answer
  • UV-A
  • UV-B
  • UV-C
  • UV-B
  • UV-A
  • UV-C
  • UV-C
  • UV-A
  • UV-B

Question 13

Question
UV-A is the least effective in killing microbes but has the deepest penetrance. UV-C is the most effective in killing microbes but has the lowes penetrance.
Answer
  • True
  • False

Question 14

Question
Hypothermiphiles and thermophiles grow in [blank_start]hot[blank_end] environments. Psychrophiles grow in [blank_start]cold[blank_end] environments. Halophiles love [blank_start]salt[blank_end]. Osmophiles love [blank_start]sugar[blank_end]. Xerophiles grow in [blank_start]dry[blank_end] environments.
Answer
  • hot
  • cold
  • salt
  • sugar
  • dry

Question 15

Question
What is true about thermophiles?
Answer
  • They love cold environments
  • They have unsaturated fatty acids
  • They have saturated fatty acids
  • They use unsaturated fatty acids to increase the melting temperature of the cell membrane.

Question 16

Question
The closer to 1, the purer the water solution.
Answer
  • True
  • False

Question 17

Question
Why do we use salt and sugar in food preservation?
Answer
  • Gives it a good flavor
  • They feed the bacteria
  • Reduces the food from looking bad
  • Reduces water activity

Question 18

Question
We can use microbes to preserve our food by them producing acid. This method of food preservation is called?
Answer
  • Fermentation
  • Salting
  • Sugaring
  • Acidification

Question 19

Question
Canned fruits naturally have higher acid than canned meat or vegetables as they have naturally occurring acidity prevents the growth of this bacteria.
Answer
  • E. coli
  • C. botulinum
  • Listeria
  • Anaerobes

Question 20

Question
What is pasteurization?
Answer
  • Heating food at a HIGH temperature for a LONG time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
  • Heating food at a LOW temperature for a SHORT time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
  • Heating food at a HIGH temperature for a SHORT time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
  • Heating food at a LOW temperature for a LONG time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food

Question 21

Question
What is true about anaerobes?
Answer
  • Boiling water to remove gas is needed to make anaerobic media.
  • Anaerobes need air to survive
  • Some obligate anaerobes have superoxide reductase
  • Obligate anaerobes can thrive in the presence of oxygen
  • They cannot utilize oxygen as an electron acceptor

Question 22

Question
Which type of organism contains a protein-free manganese complex instead of superoxide dismutase?
Answer
  • Aerobes
  • Microaerophiles
  • Aerotolerant
  • Obligate anaerobes

Question 23

Question
Aerobes and facultative aerobes always have [blank_start]superoxide dismutase[blank_end] and [blank_start]catalase[blank_end].
Answer
  • superoxide dismutase
  • catalase

Question 24

Question
Some aerotolerant organisms have [blank_start]protein-free manganese complexes[blank_end] instead of [blank_start]superoxide dismutase[blank_end]
Answer
  • protein-free manganese complexes
  • superoxide dismutase

Question 25

Question
Obligate anaerobes have [blank_start]superoxide[blank_end] [blank_start]reductase[blank_end] instead of [blank_start]superoxide[blank_end] [blank_start]dismutase[blank_end].
Answer
  • superoxide
  • reductase
  • superoxide
  • dismutase

Question 26

Question
Which of these organisms utilize oxygen as a terminal electron acceptor?
Answer
  • Aerotolerant
  • Aerobes
  • Facultative aerobes
  • Microaerophiles

Question 27

Question
Bacteriocidal [blank_start]kills[blank_end] the bacteria, bacteriostatic [blank_start]stops[blank_end] bacterial growth, and bacteriolytic [blank_start]destroys[blank_end] the cells.
Answer
  • kills
  • destroys
  • breaks down
  • stops
  • grows
  • slows
  • destroys
  • hides
  • nurse

Question 28

Question
[blank_start]Sterilants[blank_end] destroy all microorganisms including endospores [blank_start]Disinfects[blank_end] are used on surfaces to kill microorganisms but not necessarily endospores [blank_start]Sanitizers[blank_end] reduce microbial numbers but do not sterilize [blank_start]Antiseptics[blank_end] kill or inhibit microbial growth, but are non-toxic enough to be applied to living tissue
Answer
  • Sterilants
  • Disinfects
  • Sanitizers
  • Antiseptics
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