DNA (labeling) for biochem and cell biology (lecture 2)

MrSujg
Quiz by MrSujg, updated more than 1 year ago
MrSujg
Created by MrSujg about 4 years ago
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Description

1st year Biochemistry and molecular biology Quiz on DNA (labeling) for biochem and cell biology (lecture 2), created by MrSujg on 29/11/2015.
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Resource summary

Question 1

Question
Label the image
Answer
  • 1'
  • 2'
  • 3'
  • 4'
  • 5'
  • alpha
  • Beta
  • gamma
  • base

Question 2

Question
What base is it ?
Answer
  • Adenine
  • Guanine
  • Cytosine
  • Thymine
  • Uracil

Question 3

Question
What base is it ?
Answer
  • Guanine
  • Adenine
  • Cytosine
  • Thymine
  • Uracil

Question 4

Question
What base is it?
Answer
  • Cytosine
  • Adenine
  • Guanine
  • Thymine
  • Uracil

Question 5

Question
What base is it?
Answer
  • Thymine
  • Adenine
  • Uracil
  • Cytosine
  • Guanine

Question 6

Question
What base is it?
Answer
  • Uracil
  • Adenine
  • Guanine
  • Tymine
  • Cytosine

Question 7

Question
Identify primers
Answer
  • 3'
  • 5'

Question 8

Question
Label the image
Answer
  • 5'
  • 3'
  • 5'
  • 3'
  • alpha
  • beta
  • gamma
  • O
  • C
  • S
  • H
  • N
  • F
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III

Question 9

Question
Label the image
Answer
  • Minor groove
  • Major groove

Question 10

Question
Label the image
Answer
  • Guanine
  • Cytosine
  • Thymine
  • Adenine

Question 11

Question
Determine the primers
Answer
  • 5'
  • 3'
  • 3'
  • 5'
  • 3'
  • 5'
  • 5'
  • 3'

Question 12

Question
Label the image
Answer
  • A-DNA
  • B-DNA
  • Z-DNA

Question 13

Question
Label the image
Answer
  • Conservative mechanisms
  • Semiconservative mechanism

Question 14

Question
Label the image
Answer
  • Origin
  • Growing fork

Question 15

Question
Label the image
Answer
  • EcorRI restriction site
  • Origin
  • Replication bubble
  • Time of replication

Question 16

Question
Labelt the replication cartoon
Answer
  • Primase
  • SIngle0stranded region of parent DNA
  • RNA primer
  • DNA polymerase III
  • Newly made DNA
  • DNA polymerase I
  • RNA still to be replaced
  • Newest DNA
  • Completed daughter cell

Question 17

Question
Label the cartoon showing the unwinding at the origin
Answer
  • 9-mers
  • Negatively supercoiled template
  • DnaA
  • ATP
  • Initial complex
  • Open complex
  • DnaC
  • DnaB (helicase)
  • Prepriming complex

Question 18

Question
Label the cartoon showing Simultaneous synthesis on both strands
Answer
  • Point of joining
  • Lagging strand
  • Okazaki fragment
  • Short RNA primer
  • Leading strand
  • Parental DNA duplex
  • Daughter duplex
  • Direction of fork movement

Question 19

Question
Label the cartoon showing the participitation of topoisomers in DNA unwinding
Answer
  • Covalently closed circular template
  • DNA replication
  • Unwound parental duplex
  • Negative supercoils
  • Positive supercoils
  • Overwound region

Question 20

Question
Label DNA polymerase
Answer
  • Fingers
  • Thumb
  • Growing strand
  • Template strand
  • Exo
  • Palm
  • Pol

Question 21

Question
Label the image showing mismatch repair
Answer
  • Daughter strand
  • Parental strand
  • Mismatched base pair
  • Mismatch recognition
  • MutS
  • MutH
  • MutH cleavage
  • Gap repair by DNA polymerase and ligase

Question 22

Question
Label the cartoon showing excision repair
Answer
  • Initial damage recognition
  • 23B
  • XP-C
  • Opening of DNA double helix
  • TFIIH
  • XP-G
  • RPA
  • XP-F and XP-G endonucleases
  • DNA polymerase and DNA ligase

Question 23

Question
Label the image showing PCR
Answer
  • DNA Denaturation, annealing of primers
  • Elongation of primers
  • DNA denaturation; annealing or primers 2
  • Elongation of primers 2

Question 24

Question
Label the image
Answer
  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase 3

Question 25

Question
Label the image showing the Transcription in Prokaryotes
Answer
  • Promoter DNA
  • -35 sequence
  • 10 sequence
  • Coding strand
  • Template strand
  • Startpoint

Question 26

Question
Labelt the image showing A-Binding Domains (DBDs)
Answer
  • Helix-turn-helix motif
  • The lambda repressor
  • Zinc finger motif
  • Leucine zipper motif
  • Helix-loop-helix motif

Question 27

Question
Label the image showing the lac operon activation
Answer
  • Beta-galactosidase
  • RNA polymerase bound to promoter
  • lac-mRNA
  • Lactose
  • RNA polymerase transcribing the operon
  • lacI gene
  • Inactive form of repressor

Question 28

Question
Label the image showing the regulation of the lac Operon
Answer
  • Promoter
  • CAP site
  • CAP
  • a70-Pol
  • lac repressor
  • Lactose
  • cAMP

Question 29

Question
Label the image showing Eukaryotic promoters
Answer
  • GC box
  • CAAT box
  • TATA box
  • Proximal control elements
  • Core promoter
  • Start of transcription
  • Poly (A) site
  • 5' untranslated region (leader)
  • 3' untranslated region (trailer)

Question 30

Question
label the image showing General Eukaryotic Transcription Factors
Answer
  • Core promoter
  • Startpoint
  • TFIID
  • TFIIA
  • TFIIB
  • TFIIF
  • RNA polymerase 2
  • TFIIE
  • TFIIH
  • Transcription begins

Question 31

Question
Label the image showing Additional Eukaryotic Transcription Factors
Answer
  • Enhancer
  • Core promotor
  • TATA
  • Inr
  • Gene
  • Activators
  • Coactivators
  • Enhanceosome
  • TFIID
  • Activator and Coactivator
  • Transcription

Question 32

Question
label the image showing Tissue-Specific Gene Expression
Answer
  • Control elements
  • Core promoter
  • Regulatory transcription factors
  • General transcription factors
  • RNA polymerase
  • Transcription complex
  • DNA
  • RNA transcript

Question 33

Question
Label the image showing how the final mRNA is made
Answer
  • Exon
  • Intron
  • Poly (A) site
  • Termination sites
  • Transcription 5' capping
  • Cleavage at poly (A) site
  • Polyadenylation
  • RNA splicing

Question 34

Question
Labe the image showing Microarrays: Genome-Wide Analysis
Answer
  • RNA isolation
  • Reverse transcriptase labeling
  • Combine targets
  • Hybridaze to microarray

Question 35

Question
Label the image showing the molecular Dogma
Answer
  • Transcription
  • Translation
  • Assembly
  • tRNA
  • rRNA
  • Ribosomes
  • Polypeptides
  • Proteins
  • Ribosomal proteins

Question 36

Question
Label the image
Answer
  • Primary RNA transcript
  • 5'
  • 3'
  • DNA
  • Promoter
  • Transcription
  • Coding sequence
  • Upstream
  • Downstream

Question 37

Question
Follow the genetic code to match the appropriate amino acid to each codon
Answer
  • Ala
  • Cys
  • Leu
  • Arg
  • Ile
  • Val
  • Tyr
  • Glu

Question 38

Question
Label the type of the mutation
Answer
  • Frameshift
  • Missense
  • Nonsense

Question 39

Question
Identify the type of ribosome, and the size of the assembled unit
Answer
  • Prokaryotic
  • Eukaryotic (vertebrate)
  • 70S
  • 80S

Question 40

Question
Label the image showing aminoacyl-tRNAsynthetases
Answer
  • High-energy ester bond
  • Amino acid (Phe)
  • mRNA
  • Aminoacyl-tRNA
  • tRNA specific for Phe

Question 41

Question
Label the image
Answer
  • Adenosine deaminase
  • Adenosine
  • Inosine

Question 42

Question
Label the initiator methionine
Answer
  • Formyl group
  • Carboxyl group
  • Acetyl group
  • Phosphate group

Question 43

Question
Label each mRNA
Answer
  • Prokaryotic mRNA
  • Eukaryotic mRNA

Question 44

Question
Label the ribosome sites
Answer
  • A site
  • P site
  • E-site
  • mRNA-binding site
  • mRNA
  • Growing peptide chain

Question 45

Question
Label the image showing translation
Answer
  • P site
  • E site
  • A site
  • Release factor
  • free tRNA
  • Polypeptide
  • Ribosomal subunits
  • mRNA

Question 46

Question
Label the image showing translation mechanism
Answer
  • AUG start codon
  • UAG stop codon
  • mRNA
  • Initiation
  • Elongation
  • Termination
  • Release factor
  • Completed polypeptide
  • Recycling of translational components
  • Ribosomal subunits

Question 47

Question
Label the image showing western blotting
Answer
  • Electrophoresis and transfer
  • Antibody detection
  • Chromogenic detection
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