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Genome Projects and Gene Technologies

Description

AS - Level Biology (Genetics) Quiz on Genome Projects and Gene Technologies, created by Eleanor H on 03/31/2017.
Eleanor H
Quiz by Eleanor H, updated more than 1 year ago
Eleanor H
Created by Eleanor H over 5 years ago
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Resource summary

Question 1

Question
What is a genome?
Answer
  • A little creature possessing telepathic powers
  • An organism's entire genetic material
  • The entire genetic material for one gene
  • The nucleotide sequence of the introns

Question 2

Question
Why is it useful to determine the proteomes of simple organisms?
Answer
  • To create better vaccines
  • To allow monitoring of pathogens in outbreaks of disease
  • So that we can alter our own genetics to make these proteins
  • To learn more about introns

Question 3

Question
Why is it hard to translate the genome of complex organisms into their proteomes?
Answer
  • They have large sections of non-coding DNA
  • Their mRNA is hard to extract
  • They contain complex regulatory genes
  • Some genes are switched off so not all the possible proteins are made

Question 4

Question
What are the benefits of the new sequencing methods compared to the original method devised by Frederick Sanger?
Answer
  • They are automated
  • They are done on a much smaller scale so they are faster
  • They cost less to do
  • They involve less chemicals

Question 5

Question
Because DNA is [blank_start]universal[blank_end], the recipient of recombinant DNA does not have to be of the same species as the donor.
Answer
  • universal
  • complementary
  • degenerate
  • antiparallel

Question 6

Question
Making DNA fragments from mRNA 1. Extract target mRNA molecules from a cell that produces the required protein. 2. Mix the mRNA with free DNA nucleotides and [blank_start]reverse transcriptase[blank_end]. 3. The mRNA is used as a template for new strands of [blank_start]cDNA[blank_end] to be synthesised that are complementary to it.
Answer
  • reverse transcriptase
  • a restriction endonuclease
  • DNA ligase
  • DNA polymerase
  • cDNA
  • tRNA
  • rRNA
  • miRNA

Question 7

Question
[blank_start]Restriction Endonucleases[blank_end] digest the DNA at specific [blank_start]recognition[blank_end] sequences. These sites are special because they are [blank_start]palindromic[blank_end], meaning that they consist of [blank_start]antiparallel[blank_end] base pairs. Some types of this enzyme cut the DNA so that small tails of unpaired bases are created at each end of the fragment. These are called [blank_start]sticky[blank_end] ends and can be used to [blank_start]anneal[blank_end] DNA fragments together as long as the ends are complementary.
Answer
  • Restriction Endonucleases
  • Reverse Transcriptases
  • recognition
  • palindromic
  • antiparallel
  • sticky
  • blunt
  • anneal
  • recconect
  • digest
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