Recombination and transformation

Recombination and Transformation

Restriction Endonucleases cut the gene of intrest from human DNA, and cut open the plasmid

DNA Ligase joins the DNA sequences with the insulin gene inputed in the plasmid

The Plasmid is inserted into the bacteria by heat shock or electrophortion

Bacteria placed on an antibiotic to determine whether they have been taken up by the plasmid.

Insulin Genes are inserted next to a gene, coding for Beta Galactosidose (Blue protein). Transformed bactera glow blue for easy identification and harvesting.

This process is completed in seperate bacteria for chain A and B. Subsequent proteins made by bacteria are joined together.

Stage 1

Stage 2

Stage 3

Stage 4

Stage 5

Stage 6