what effects enzyme activity

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There are a couple of factors affecting enzyme activity:

•temperature

=>higher temperature means higher kinetic energy for both

enzyme molecules and substrate molecules this means that collisions are more

frequent so chances of enzyme-substrate complex forming larger however too high a temperature disrupts the bonds

that give the enzyme its tertiary structure hence the active site no longer

fits the substrate the enzyme is denatures

=>low temperature

slows down enzyme reaction eventually having the same effect as high

temperature, change tertiary structure enzyme is denatured

•pH- again affects the bonds in the tertiary structure of

the enzyme and so will change active site shape and denature enzyme

•increased concentration of enzyme means larger number of

enzymes in the same space more frequent collision due to random movement so

larger number of enzyme-substrate complexes can be formed

•increased substrate concentration has the same effect as

increased enzyme concentration

•if the enzyme needs a cofactor to facilitate the reaction

then that cofactor acts by helping the formation of that enzyme-substrate

complex

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