DNA (labeling) for biochem and cell biology (lecture 2)

Beschreibung

1st year Biochemistry and molecular biology Quiz am DNA (labeling) for biochem and cell biology (lecture 2), erstellt von MrSujg am 29/11/2015.
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Quiz von MrSujg, aktualisiert more than 1 year ago
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11

Zusammenfassung der Ressource

Frage 1

Antworten
  • 1'
  • 2'
  • 3'
  • 4'
  • 5'
  • alpha
  • Beta
  • gamma
  • base

Frage 2

Frage
What base is it ?
Antworten
  • Adenine
  • Guanine
  • Cytosine
  • Thymine
  • Uracil

Frage 3

Frage
What base is it ?
Antworten
  • Guanine
  • Adenine
  • Cytosine
  • Thymine
  • Uracil

Frage 4

Frage
What base is it?
Antworten
  • Cytosine
  • Adenine
  • Guanine
  • Thymine
  • Uracil

Frage 5

Frage
What base is it?
Antworten
  • Thymine
  • Adenine
  • Uracil
  • Cytosine
  • Guanine

Frage 6

Frage
What base is it?
Antworten
  • Uracil
  • Adenine
  • Guanine
  • Tymine
  • Cytosine

Frage 7

Frage
Identify primers
Antworten
  • 3'
  • 5'

Frage 8

Antworten
  • 5'
  • 3'
  • 5'
  • 3'
  • alpha
  • beta
  • gamma
  • O
  • C
  • S
  • H
  • N
  • F
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III

Frage 9

Antworten
  • Minor groove
  • Major groove

Frage 10

Antworten
  • Guanine
  • Cytosine
  • Thymine
  • Adenine

Frage 11

Frage
Determine the primers
Antworten
  • 5'
  • 3'
  • 3'
  • 5'
  • 3'
  • 5'
  • 5'
  • 3'

Frage 12

Antworten
  • A-DNA
  • B-DNA
  • Z-DNA

Frage 13

Antworten
  • Conservative mechanisms
  • Semiconservative mechanism

Frage 14

Antworten
  • Origin
  • Growing fork

Frage 15

Antworten
  • EcorRI restriction site
  • Origin
  • Replication bubble
  • Time of replication

Frage 16

Frage
Labelt the replication cartoon
Antworten
  • Primase
  • SIngle0stranded region of parent DNA
  • RNA primer
  • DNA polymerase III
  • Newly made DNA
  • DNA polymerase I
  • RNA still to be replaced
  • Newest DNA
  • Completed daughter cell

Frage 17

Frage
Label the cartoon showing the unwinding at the origin
Antworten
  • 9-mers
  • Negatively supercoiled template
  • DnaA
  • ATP
  • Initial complex
  • Open complex
  • DnaC
  • DnaB (helicase)
  • Prepriming complex

Frage 18

Frage
Label the cartoon showing Simultaneous synthesis on both strands
Antworten
  • Point of joining
  • Lagging strand
  • Okazaki fragment
  • Short RNA primer
  • Leading strand
  • Parental DNA duplex
  • Daughter duplex
  • Direction of fork movement

Frage 19

Frage
Label the cartoon showing the participitation of topoisomers in DNA unwinding
Antworten
  • Covalently closed circular template
  • DNA replication
  • Unwound parental duplex
  • Negative supercoils
  • Positive supercoils
  • Overwound region

Frage 20

Frage
Label DNA polymerase
Antworten
  • Fingers
  • Thumb
  • Growing strand
  • Template strand
  • Exo
  • Palm
  • Pol

Frage 21

Frage
Label the image showing mismatch repair
Antworten
  • Daughter strand
  • Parental strand
  • Mismatched base pair
  • Mismatch recognition
  • MutS
  • MutH
  • MutH cleavage
  • Gap repair by DNA polymerase and ligase

Frage 22

Frage
Label the cartoon showing excision repair
Antworten
  • Initial damage recognition
  • 23B
  • XP-C
  • Opening of DNA double helix
  • TFIIH
  • XP-G
  • RPA
  • XP-F and XP-G endonucleases
  • DNA polymerase and DNA ligase

Frage 23

Frage
Label the image showing PCR
Antworten
  • DNA Denaturation, annealing of primers
  • Elongation of primers
  • DNA denaturation; annealing or primers 2
  • Elongation of primers 2

Frage 24

Antworten
  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase 3

Frage 25

Frage
Label the image showing the Transcription in Prokaryotes
Antworten
  • Promoter DNA
  • -35 sequence
  • 10 sequence
  • Coding strand
  • Template strand
  • Startpoint

Frage 26

Frage
Labelt the image showing A-Binding Domains (DBDs)
Antworten
  • Helix-turn-helix motif
  • The lambda repressor
  • Zinc finger motif
  • Leucine zipper motif
  • Helix-loop-helix motif

Frage 27

Frage
Label the image showing the lac operon activation
Antworten
  • Beta-galactosidase
  • RNA polymerase bound to promoter
  • lac-mRNA
  • Lactose
  • RNA polymerase transcribing the operon
  • lacI gene
  • Inactive form of repressor

Frage 28

Frage
Label the image showing the regulation of the lac Operon
Antworten
  • Promoter
  • CAP site
  • CAP
  • a70-Pol
  • lac repressor
  • Lactose
  • cAMP

Frage 29

Frage
Label the image showing Eukaryotic promoters
Antworten
  • GC box
  • CAAT box
  • TATA box
  • Proximal control elements
  • Core promoter
  • Start of transcription
  • Poly (A) site
  • 5' untranslated region (leader)
  • 3' untranslated region (trailer)

Frage 30

Frage
label the image showing General Eukaryotic Transcription Factors
Antworten
  • Core promoter
  • Startpoint
  • TFIID
  • TFIIA
  • TFIIB
  • TFIIF
  • RNA polymerase 2
  • TFIIE
  • TFIIH
  • Transcription begins

Frage 31

Frage
Label the image showing Additional Eukaryotic Transcription Factors
Antworten
  • Enhancer
  • Core promotor
  • TATA
  • Inr
  • Gene
  • Activators
  • Coactivators
  • Enhanceosome
  • TFIID
  • Activator and Coactivator
  • Transcription

Frage 32

Frage
label the image showing Tissue-Specific Gene Expression
Antworten
  • Control elements
  • Core promoter
  • Regulatory transcription factors
  • General transcription factors
  • RNA polymerase
  • Transcription complex
  • DNA
  • RNA transcript

Frage 33

Frage
Label the image showing how the final mRNA is made
Antworten
  • Exon
  • Intron
  • Poly (A) site
  • Termination sites
  • Transcription 5' capping
  • Cleavage at poly (A) site
  • Polyadenylation
  • RNA splicing

Frage 34

Frage
Labe the image showing Microarrays: Genome-Wide Analysis
Antworten
  • RNA isolation
  • Reverse transcriptase labeling
  • Combine targets
  • Hybridaze to microarray

Frage 35

Frage
Label the image showing the molecular Dogma
Antworten
  • Transcription
  • Translation
  • Assembly
  • tRNA
  • rRNA
  • Ribosomes
  • Polypeptides
  • Proteins
  • Ribosomal proteins

Frage 36

Antworten
  • Primary RNA transcript
  • 5'
  • 3'
  • DNA
  • Promoter
  • Transcription
  • Coding sequence
  • Upstream
  • Downstream

Frage 37

Frage
Follow the genetic code to match the appropriate amino acid to each codon
Antworten
  • Ala
  • Cys
  • Leu
  • Arg
  • Ile
  • Val
  • Tyr
  • Glu

Frage 38

Frage
Label the type of the mutation
Antworten
  • Frameshift
  • Missense
  • Nonsense

Frage 39

Frage
Identify the type of ribosome, and the size of the assembled unit
Antworten
  • Prokaryotic
  • Eukaryotic (vertebrate)
  • 70S
  • 80S

Frage 40

Frage
Label the image showing aminoacyl-tRNAsynthetases
Antworten
  • High-energy ester bond
  • Amino acid (Phe)
  • mRNA
  • Aminoacyl-tRNA
  • tRNA specific for Phe

Frage 41

Antworten
  • Adenosine deaminase
  • Adenosine
  • Inosine

Frage 42

Frage
Label the initiator methionine
Antworten
  • Formyl group
  • Carboxyl group
  • Acetyl group
  • Phosphate group

Frage 43

Antworten
  • Prokaryotic mRNA
  • Eukaryotic mRNA

Frage 44

Frage
Label the ribosome sites
Antworten
  • A site
  • P site
  • E-site
  • mRNA-binding site
  • mRNA
  • Growing peptide chain

Frage 45

Frage
Label the image showing translation
Antworten
  • P site
  • E site
  • A site
  • Release factor
  • free tRNA
  • Polypeptide
  • Ribosomal subunits
  • mRNA

Frage 46

Frage
Label the image showing translation mechanism
Antworten
  • AUG start codon
  • UAG stop codon
  • mRNA
  • Initiation
  • Elongation
  • Termination
  • Release factor
  • Completed polypeptide
  • Recycling of translational components
  • Ribosomal subunits

Frage 47

Frage
Label the image showing western blotting
Antworten
  • Electrophoresis and transfer
  • Antibody detection
  • Chromogenic detection
Zusammenfassung anzeigen Zusammenfassung ausblenden

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