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Carotenos 2º método

Jorge Revilla Spanish

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  1. Inicio

  2. 5 mg de mustra liofilizada

  3. 1mL MeOH/Cl3CH/ tris-HCl (1:2:1)

  4. Extraemos

  5. Añadimos alfa-tocoferol como patrón interno

  6. Centrifugar y recuper la fase orgánica

  7. Speed vac

  8. Redisolver en 100 uL de acetato de etilo

  9. Analizar empleando UPLC-PDA

  10. Fin

  11. La longitud de onda es 285nm

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carotenos
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Jorge Revilla
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