Flashcards
3-1
Cards in this deck (11)
- 1Clarity of an image is calledresolution
- 2an actual measurement of how far apart two points must be for the microscope to view them as being separate.limit of resolution
- 3the resolution improves as the limit of resolution is made ....smaller
- 4the best limit of resolution achieved by a light microscope0.2 um
- 5Look at formulas on page 143h
- 6the measure of a lens's ability to capture light coming from the specimen and use it to make the imagenumerical aperture
- 7a special condenser that is used so only the light reflected off the specimen enters the objectivedark-field microscopy
- 8this uses special optical components to exploit subtle differences in the refractive indices of water and cytoplasmic components to produce contrastphase contrast microscopy
- 9this uses a fluorescent dye that emits fluorescence when illuminated with ultraviolet radiationfluorescence microscopy
- 10assuming that all other variables remain constant, explain why light of shorter wavelengths will produce a clearer image than light of longer wavelengthsa smaller wavelength will allow for a smaller D value. a small D value means better resolution so it would be more clear.
- 11the NA of the condenser and low-power objective lenses are 1.25 and 0.25. and it has a wavelength of 520 nm.Look at page 150
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- Biolab Flashcards on 3-1, created by Megan Coeur on 21/04/2018.
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- Megan Coeur
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