GoConqr has a new look! Let us know what you think.

Sign up free

Flashcards

Lab Midterm Practical

Diamond Sapphire English 53 cards

Lab Midterm Practical

Cards in this deck (53)

  1. 1—Bile Esculin AgarBile Esculin Agar
  2. 2—Blood AgarBlood Agar
  3. 3—Citrate AgarCitrate Agar
  4. 4—Columbia CNA w/ 5% sheep blood agarColumbia CNA w/ 5% sheep blood agar
  5. 5—Eosin Methylene Blue AgarEosin Methylene Blue Agar
  6. 6—DNaseDNase
  7. 7—Methyl Red and Voges-ProskauerMethyl Red and Voges-Proskauer
  8. 8—Mannitol Salts AgarMannitol Salts Agar
  9. 9—MacConkey AgarMacConkey Agar
  10. 10—Casein hydrolysisCasein hydrolysis
  11. 11—Lysine Iron AgarLysine Iron Agar
  12. 12—Triple Sugar Iron AgarTriple Sugar Iron Agar
  13. 13—SIM (Sulfur Indole Motility)SIM (Sulfur Indole Motility)
  14. 14—Urea HydrolysisUrea Hydrolysis
  15. 15—Starch HydrolysisStarch Hydrolysis
  16. 16—Xylose Lysine DesoxycholateXylose Lysine Desoxycholate
  17. 17Columbia CNA w/ 5% sheep bloodSelective for gram positives
    presence of blood allows for differentiation based on hemolysis of RBCs
  18. 18CNA: 1. P/NG 2. G/beta hemolysis 3. G/alpha hemolysis 4. G/gamma hemolysis1. probable gram negative
    2. probable beta-hemolytic staph, strep, or enterococcus
    3. probable alpha-hemolytic staph, strep, or enterococcus
    4. probable gamma-hemolytic staph, strep, or enterococcus
  19. 19Bile Esculin TestSelects for intestinal organisms
    differential: presence of esculinase enzyme
  20. 20BEA: 1. medium darkened 2. no darkening1. presumptive ID of intestinal organism. Esculin+acid=esculetin which reacts with iron to create dark brown colour
    2. presumptive determination as not an intestinal organism
  21. 21Mannitol Salt AgarSelective for staphylococci
    differential: phenol red indicator
  22. 22MSA: 1. P/NG 2. G/Y 3. G/R1. not staphylococcus (inhibited by NaCl)
    2. possible s. aureus (mannitol fermentation)
    3. staph other than s. aureus (no fermentation)
  23. 23MacConkeySelective for gram negative
    differential: neutral red dye
  24. 24MAC: 1. P/NG 2. G/R or P 3. G/C1. gram positive
    2. probable coliform (lactose fermenter)
    3. noncoliform (no fermentation)
  25. 25Eosin Methylene BlueSelective for gram negative
    differential: dyes + lactose fermenters
  26. 26EMB: 1. P or NG 2. G/Pi 3. G/D 4. G/C1. gram positive
    2. possible coliform (ferments lactose w/ little acid production)
    3. probable coliform (ferments lactose and/or sucrose w/ acid production)
    4. noncoliform (no fermentation)
  27. 27Xylose Lysine Desoxycholateselective for gram negative
    differential: H2S production; phenol red pH indicator
  28. 28XLD: 1. P or NG 2. G/Y 3. G/RB 4. G/R1. Gram positive
    2. xylose fermentation
    3. reduces sulfur; no xylose fermentation or xylose fermentation with lysine decarboxylation
    4. no xylose fermentation or xylose fermentation with lysine decarboxylation; no sulfur reduction;
  29. 29—Oxidation-Fermentation TestOxidation-Fermentation Test
  30. 30Oxidation-Fermentationused to differentiate bacteria based on their ability to oxidize or ferment specific sugars. (oil creates anaerobic environment)
  31. 31OF test: 1.Sealed: G/B; unsealed: any Y 2. Sealed: Y; unsealed: Y 3. sealed: Y at top; unsealed: Y at top 4. sealed: G/B; unsealed: G/B1. Oxidation
    2. Oxidation and fermentation or fermentation only
    3. Oxidation and slow fermentation or slow fermentation only
    4. no sugar metabolism; nonsaccharolytic organism
  32. 32Methyl-Red and Voges-Proskauer Testsused to distinguish between members of the family Enterobacteriaceae and to differentiate between other gram negative rods
  33. 33Methyl Red Results: 1. media turns red immediately 2. no colour change1. Mixed acid fermentation occurs
    2. no mixed acid fermentation
  34. 34Voges-Prokskaeur results: 1. Red colour 2. No colour change1. 2,3-butanediol fermentation (acetoin produced)
    2. no 2,3-butanediol fermentation
  35. 35Oxidase test: 1. blue colour w/in 20s 2. no colour change w/in 20s1. presence of cytochrome c oxidase
    2. no cytochrome c oxidase
  36. 36citrate testused to determine the ability of an organism to use citrate as its sole source of carbon. Distinguishes between Enterobacteriaceae and differentiate from other gram negative rods
  37. 37Citrate test: 1. any blue colour 2. no colour change1. citrate is utilized (citrate-permease present)
    2. no citrate utilized
  38. 38Starch Hydrolysisaids in differentiating members of the genera corynebacterium, clostridium, bacillus, bacteroides, fusobacterium, and enterococcus
  39. 39Starch hydrolysis: 1. clearing of media after iodine added 2. no clearing of media after iodine added1. organism can produce alpha-amylase and hydrolyze starch
    2. organism cannot hydrolyze starch (no alpha-amylase)
  40. 40Urea Hydrolysisused to differentiate organisms based on their ability to hydrolyze urea via urease. Helps identify Proteus genera
  41. 41Urea hydrolysis: 1. all pink (24h and 24+h) 2. partially pink (24h); all pink or partially pink (24+h) 3. orange or yellow (24h); all or partially pink (24+h) 4. orange or yellow (24h and 24+ h)1. rapid urea hydrolysis
    2. slow/weak urea hydrolysis
    3. slow/weak urea hydrolysis
    4. no urea hydrolysis
  42. 42Casein Hydrolysis 1. Clearing 2. No clearingDifferential: Presence or absence of Casease
    1. Casease present
    2. Casease absent
  43. 43DNA Hydrolysis 1. Clearing 2. No clearingDifferential: Presence or absence of DNase. Differentiate Serratia from Enterobacter, S. aureus from other Staphylococcus species
    1. DNase present
    2. DNase absent
  44. 44SIM MediumCombo differential
    Sulfur, Indole, Motility
    Cysteine desulfurase and thiosulfate reductase (H2S byproduct)..differentiate members of enterobacteriaceae
    Indole from tryptophanase (check with Kovac's reagent)
  45. 45TSI AgarTriple Sugar Iron
    Differentiate members of Enterobacteriaceae and distinguish them from other gram negative rods
    H2S production and gas production also tested
  46. 46TSI Agar: 1. A/A 2. K/A 3. K/K 4. K/NC 5. NC/NC1. Glucose and lactose and/or sucrose fermentation with acid accumulation in slant and butt
    2. Glucose fermentation with acidic by products; aerobic peptide catabolization with alkaline products in slant (reversion)
    3. No fermentation of sugars; aerobic and anaerobic peptide catabolism producing alkaline byproducts
    4. no fermentation; peptide catabolism only aerobically with alkaline products
    5. organism not growing or growing slowly
  47. 47LIA agarLysine Iron Agar
    differentiate enteric based their ability to decarboxylate or dominate lysine and product H2S.
  48. 48LIA: 1. K/K 2. K/A 3. R/A1. lysine carboxylase positive (alkaline products)
    2. glucose fermentation; no carboxylation or deamination; aerobic peptone degradation resulting in alkaline products in slant
    3. deaminase positive; glucose fermentation. deamination (only aerobic) reacts with ferric ammonium citrate to product red colour
  49. 49MPN testmost probable number of fecal coliforms in a water sample by lactose fermentation of organisms in a sample
    also called "multiple tube fermentation method"
  50. 50LTB tubesLaurel Tryptose Broth
    selective for: coliform groups (but does not screen out non-coliforms); simply used to test for presence or absence of coliforms
    part of MPN/multiple fermentation tube test
  51. 51BGLB tubesBrilliant Green Lactose Bile
    Selects against: non-coliforms; confirms presence of coliforms
    part of MPN/multiple fermentation tube test
  52. 52EC tubesselective for: E. coli (when incubated at 45.5ºC)
    part of MPN/multiple fermentation tube test
  53. 53MPN/100 mL=100P/[sqrt(VaVn)]
    P=total # of + results (BGLB or EC)
    Va=combined volume of sample in all LTB tubes
    Vn=combined volume of sample in LTB tubes that produced - results in BGLC or EC

Information

Description
Flashcards on Lab Midterm Practical, created by Diamond Sapphire on 28/02/2019.
Views
0
Rating

Feedback

What is it about?

What do you think of the new look?

How do you like it?