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Flowchart

N-glycan release and LC-MS/MS glycomics of lactoferrin

maria jose saucedo Spanish

Flowchart content

  1. Purified human and bovine lactoferrin

  2. Lactoferrin reduction

  3. Glycoprotein alkylation

  4. Alkylation quenching

  5. Deamination of N-linked glycans

  6. N-linked glycans reduction

  7. SCX microcolumns preparation

  8. Desalting reduced N-linked glycans

  9. Glycomics using LC-MS/MS

  10. Release of N-linked glycans with PNGase F

  11. Purified human and bovine lactoferrin

  12. Lactoferrin reduction

  13. Glycoprotein alkylation

  14. Alkylation quenching

  15. N-linked glycans release (PNGase F)

  16. N-linked glycans deamination

  17. N-linked glycans reduction

  18. SCX microcolumns preparation

  19. Desalting reduced N-linked glycans

  20. Glycomics using LC-MS/MS

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Description
Flowchart on Untitled, created by maria jose saucedo on 27/10/2017.
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