Chromatography

Chromatography

1. Fluid entering column is ELUENT

2. Column

   • Packed: filled with particles of S Phase
   • Open Tubular: narrow, hollow capillary with S Phase coated on inside walls
   • Avoid introducing particular matter because columns is expensive and degraded, samples should be centrifuged/filtered through 0.5um by manual injection
   • Heating decreases viscosity
     • Reduces pressure and gets faster flow
     • Increasing temp can degrade the S Phase and decrease column life

3. Flow

   • Linear: Distance traveled in 1 min by solvent cm
   • Volume: mL traveled by solvent per min