Chromatography (2)

Chromatography (2)

1. Key Terms

   • Eluent
     • Fluid entering a column
   • Eluate
     • Fluid which comes out of a column
   • Elution
     • A fluid which is passed through a gas or liquid chromatography column
   • Solute
     • A singles component of a mixture

2. Uses

   • Chromatography can be used for qualitative analysis
   • Quantitative anaysis
   • Purify substances

3. Chromatogram

   • This is a graph which shows the time taken for the mobile phase and all separated components from injection to detector

4. Mathematics

   • Retention Time (tR)
     • The time spent by the component within the column
   • Retention Volume
     • The volume of mobile phase needed to elute a compnent from the column
     • Calculated by multiplying retention time by flow rate of mobile phase
   • Mobile phase hold up time (tm)
     • The time taken for the mobile phase and any unretarded components to pass through the system
   • Peak WIdth (Wb)
     • The width of a peak at the base
   • Peak Height
     • The width of a peak at 50% height
   • Theoretical Plate Number (N)
   • Capacity Factor
   • Resolution
     • The distance between the centre of the two peaks divided by the average of the two peaks
   • Asymmetrical Factor (AF)
   • HETP

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