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Midterm 2 Quiz
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Questions in this quiz (18)
1What transfers out of the bag during dialysis
- Abuffers
- Bsalts
- Cwater
- Dions
- Ecations but not anions
- Fanions but not cations
- Glarger parts of protein
- Hparts of protein where PI (protein) >Ph (buffer)
2Gel filtration separates proteins by [blank_start]size[blank_end].
- Asize
3Ion exchange chromatography separates protein(s) by [blank_start]charge[blank_end].
- Acharge
4Do larger or smaller proteins/parts of protein flow through gel filtration first?
- Alarger
- Bsmaller
5Anion exchanger means that it removes [blank_start]anions[blank_end] from protein mixture so that means the resin must be decorated with [blank_start]positively[blank_end] charged moieties
- Aanions
- Bpositively
6What type of protein purification would I use if I had a wide range of charges within my protein and I know only the charge of the protein that I want.
- ASDS Page
- BDialysis
- CGel Filtration
- DIon Exchange
- EIsoelectric Focusing Electrophoresis
7When performing Cation Exchanger Ion Exchange Chromatography, which proteins will be removed first when eluding: answer in one word: [blank_start]blank[blank_end]
- Apositive
8Weakly charged proteins are knocked off last when eluding using Ion Exchange Chromatography.
- ATrue
- BFalse
9Affinity Chromatography can only separate proteins by size.
- ATrue
- BFalse
10In gel electrophoresis, anions travel to the [blank_start]anode[blank_end].
- Aanode
11Paper chromatography is most commonly used to separate which of the following:
- ALarge proteins
- BEnzymes
- CAmino acids
- DPeptides of differing charge
- ESmall proteins
- FOpposingly charged proteins
12SDS Page separates proteins based on [blank_start]size[blank_end].
- Asize
13What is the purpose of using SDS (sodium dodecylsulfide)?
- Adenature proteins
- Bbreak disulfide bonds
- Cgive molecules uniform charge/mass ratio
- Dseparate proteins based on PI
14What is the purpose of using BME?
- ABreak disulfide bonds
- BCreate disulfide bonds
- CDenature proteins
- DProvide linear chain for protein
15Separates proteins using PI?
- AAffinity Chromatography
- BIon exchange Chromatography
- CIsoElectric Focusing
- DDialysis
- ESalting out
16The protein's charge will increase as it goes down the column for IsoElectric Focusing.
- ATrue
- BFalse
17When does the protein stop traveling in IsoElectric Focusing electrophoresis
- AWhen pH>pI
- Bwhen pH region corresponds to pI
- Cwhen pH<pI
- Dwhen net charge = 1
18What comes first in 2d gel electrophoresis
- AIEF Gel
- BSDS PAGE
- CWestern Blotting
- DNative PAGE
- EBME Purification
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- AP Biochem Quiz on Midterm 2 Quiz, created by Arnav Garg on 11/10/2023.
- Author
- Arnav Garg
- Language
- English
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