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Microscopy
Fichas del conjunto (28)
- 12 components of stainschromogen & auxochrome
- 22 types of stainsacidic & basic
- 3edescribe: acidic dyeviews (+)-ly chraged cell components (proteins); e.g.: nigrosin, eiosin Indian ink
- 4describe: basic dyeviews the (-)-ly charged cell components (DNAA, ccell walls); e.g.: methylene blue, safranin, crystal violet; more common
- 5type of stain which differentiates Clostridium from BacillusEndospore stain
- 6stain used to bacteria which cannot undergo Gram stainingAcid-Fast stain
- 7regent used in Acid Fast stainingcarbol fuschin
- 8e.g. of organism for Acid Fast stainMycobacterium
- 9reason for bright bg for Brightfield Microscopytransmitted light is absorbed
- 10rationale: Phase-contrast microscopydiff. in phases & WL of light transmitted and light reflected
- 11special component in Phase-Contrast microscopeannular stop
- 12reason for dark bg for Darkfield Microscopytransmitted light not absorbed
- 13special thing about Fluorescent microscopeFluorophore
- 14rationale: Fluorescent microscopeconversion of a long WL to a shorter WL
- 15source of intense beam in fluorescent microscopemercury vapor arc lamp
- 16rationale: Differential-Interference Contrast Microscopysource of light: 2 light beams perpendicular at each other
- 17special equipment use din Atomic Force Microscopycantilever and laser
- 18rationale: Confocal Scanning Laser Microscopyuses a laser as light source, surface detected by sensor, generates 3d image
- 19~WL used in EM0.005 nm
- 20compare: SEM vs TEMSEM: 3D image; e- on the surface
TEM:higher resolution, 2D image, e- transmitted - 21equipment used to physically break whole cellsDounce homogenizer
- 22what is prostheca?feet-like projection in Caulobacter
- 233 importance of shape- variety with uniformity, active modification of shapes, evolutionary progress
- 242 largest bacteriaEpulopiscium fishelsoni
Thiomargarita nambiensis - 252 smallest bacteriaMycoplasma pneumoniae
Pelagibacter - 26explain: the Goldilocks theoryprotozoan predators prefer intermediate-sized bacteria as preys
- 273 reasons why bacteria are small- theoretical limits, SA/V ratio, intracellular mixing
- 28compare: Archaea vs Bacteria & Eukarya cell membraneA: L-glycerol, ether-linkage, branched isoprene chains
B&E: D-glyccerol, ester-linkage, unbranched fatty acid chains
Información
- Descripción
- From Lecture 2: Cellular Structure & Function
- Creado por
- Nicole Bolo
- Visitas
- 28
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