Fichas
Βacillus
Fichas del conjunto (29)
- 1What are the microscopical shape and size of Bacillus?large rods- 10μm
motile - 2Name the 2 staining methods for bacillus and their results1. gramm staining= +
2. wirtz-conclin= green endospores and the rest red. - 3Cultivation method for bacillus:* MPBA
* 37°C for 1-2 days
* Aerobes/facultative anaerobic - 4Which animals are the most susceptible to infection by B. Anthracis?Ruminants
- 5Which form of B. Anthracis is the most common?The cutaneous form- 95%
- 6Name 3 forms of B. Anthracis:1) Cutaneous
2)Pulmonary
3) GIT form - 7Which bacillus causes a dangerous zoonotic disease? What is the name of the disease?B. Anthracis causes Anthrax, splenic fever
- 8What are the results of Catalase and oxidase test for bacillus?Cat +
Ox - - 9What is the name of the test that is used for detecting anthrax ?Ascoli reaction/ precipitation test
Adding antigen on top of antiserum with Ab’s and the result is a ring in the middle - 10Which bacillus is positive for ascoli and perl test, and which is negative?+ : B. Anthracis
- : B. Cereus - 11Which strain causes hemolysis? What type?B. Cereus- β haemolysis
- 12All strains of bacillus are motileNope
Only B. Cereus is
(B. Anthracis is non-motile) - 13None of the strains have capsulaB. Anthracis has a capsule
- 14In order to prevent the spread of anthrax from dead bodies it is necessary to:Bury the carcasses deep down or keep it at 150°C for 3h.
The cell wall of the bacteria breaks when heated. - 15What is typical for pulmonary form of B. Anthracis?Chest edema followed with cardiovascular shock.
High mortality - 16High temperature and eschars are typical for:Cutaneous form of B. Anthracis
Causes black necrotic lesions aka eschars - 17The less common form of B. Anthracis which however is fatal after ingesting the spores is:GIT form
- 18** bonus question :) ** Which bacillus was used as a biological weapon for terrorist attacks and when??B. Anthracis in 2001, USA office attacks
- 19The toxins of B. anthracis in general cause:swelling, necrosis, septicemia
all will lead to increased vascular permeability, hemorrhages and death. - 20Zinc metalloprotease is produced by____and causes____ :B. Anthracis
death of macrophages - 21Which strain produces enterotoxins? What do they cause?B. Cereus
Will cause alimentary intoxication, gastritis, enteritis, vomiting and diarrhea - 22Which strain possess a β-lactamase gene? What does this mean for the bacterium?B. Anthracis
When the gene is activated the becterium will become resistan to β-lactam antibiotics making it more difficult to treat. - 23What test is used for detection of enterotoxins?ELISA
- 242 not so important strains of bacillus are:B. Subtillis- mastitis
B. Larvae- plague of bees fetus - 25What is the process for wirtz-conclin staining?• Heat fixation on slide
• 5% malachite green
• Heat
~~Repeat green and heat 3 times~~
• Wash with water
• Carbolfuchsin staining - 26What is one factor that gives the bacteria their increased resistance?Sporogenic: produce spores which give them increased resistance
- 27What happens in splenic fever?The spleen is destroyed and there are problems with circulation
- 28Is splenic fever transmissible?YES
Usually from CATTLE to human from SKIN= cutaneous/skin transmissible. Causes sepsis (blood intoxication), increased temp., necrotic skin lesions - 29Which strain is opportunistic?B. Cereus
Also survives heat (heat treatment in food) and so it's an important contaminant of food
Información
- Descripción
- Microbiology Fichas sobre Βacillus, creado por Sotia P. el 14/04/2019.
- Creado por
- Sotia P. · abr 2019
- Actualizado
- 14 abr 2019
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