Cell Structure & Analysis

Cell Structure & Analysis

1. Eukaryotic Cells

   • Have a Nucleus
   • Organelles
     • Nucleus
     • Ribosomes
     • Mitochondria
     • Smooth ER
     • Rough ER
     • Nucleolus
     • Lysosomes
     • Golgi appratus
     • Vessicles
     • Cytoplasm
     • Chloroplasts - Plant and Algal cells
     • Cell wall - Plant, Algal & Fungal cells
     • Cell membrane

2. Prokaryotic Cells

   • No Nucleus
   • Organelles
     • Plasmids
     • Cytoplasm
     • Ribosomes
     • Cell wall
     • Cell membrane
     • Capsule (Some)
     • Pilus
     • Flagella
     • Free floating DNA

3. Analysis Of Cells

   • Conditions
     • Isotonic - Maintain water potential
       • Prevent organelles bursting or shrinking
     • Buffer - Maintain Ph
     • Ice Cold - prevent/ lower enzyme activity
     • Filtration - Remove any debris
     • Homogenisation - Release organelles
   • Microscopes
     • Optical
       • Low magnification
       • Low Resolution
       • Cheap
     • Electron
       • Scanning
         • 3D Image
         • Thick specimen
         • Lower Resolution than Transmission
         • Scan beam of electrons across sample
       • Tranmission
         • Higher Resolution
         • Thin specimen
         • Dead samples only
         • Beam of focused electrons

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