Chromatography

Página 1

Chromatography

1. Place sample n a pencil line drawn a few centimatres from the bottom of the chromatography paper

2. Hang or place paper vertically dipped into solvent (generally water, but for amino acids butan-1-ol, glacial ethanoic acid, and water, ensuring it does not cover the sample

3, Different solutes, will have different levels of solubility and there for attraction to the solvent vs attraction to the paper, this means they will ascend at different rates

4. the solvent reaching point is the solvent front

5.

For amino acids only, spray ninhidrin ina fume cupboard to make them visible

Página 2