CALU-3 - created from Mind Map

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HISTORY (details of donor)

CULTURE CONDITIONS

TYPE OF CELL LINE

GROWTH CHARACTERISTICS

SPECIES

DEFINITION

Name of depositor: J Fogh Deposited as: Homo sapiens Age: 25 years Gender: male Ethnicity: Caucasian Disease: adenocarcinoma The patient had received prior therapy with cytoxan, bleomycin and adriamycin. Blood Type A; Rh+

subculture recommendations

method of dissociation

supplements

culture medium

mode of growth

maintained in a 1:1 mixture of Ham's F12, DMEM, 10% fetal bovine serum supplemented with 100 μg/ml penicillin G (Sigma), 100 μg/ml streptomycin sulfate (Sigma), cells were plated in 150 cm2cell culture flasks and subcultured before reaching confluency using a 0.25% trypsin solution in EDTA, culture medium was changed every 2 days, cells were split 1:2 during each passage.

Well-differentiated human bronchial cell line with the characteristics of the serous cells of airway submucosal glands. The submucosal glands play a major role in mucociliary clearance because they secrete electrolytes that facilitate airway hydration.

Calu-3 cells were grown using air-interfaced condition (AIC) on polyester cell culture supports. Monolayers were evaluated for transepithelial electrical resistance (TEER), permeability to the paracellular marker fluorescein sodium (flu-Na), surface P-gp expression, and functionality. Mucous secretion was also identified by alcian blue staining

put them in a medium with higher serum concentration

have characteristics of both serous and mucus cells, can be cultured as a flat sheet, and respond to secretagogues that regulate the glands in vivo. They form polarized monolayers with tight junctions and express several acinar cell markers.

continuous

transformed

growth rate

morphology

plating efficiency

cell viability

continuously propagated, grew rapidly and consistently, and were passaged approximately once a week

monolayers, cuboidal and polygonal cells, microvilli on the apical surface

Let the cells grow on the top of feeder cells. Plate irradiated 3T3 cells the day before plating Calu3 cells. They grow much better .

It is based on cellular mitochondrial dehydrogenase (MDH) activity, which measured by MTT reduction and conversion to blue formazan salt that was quantified after extraction from cells.

Homo sapiens

a human lung cancer epithelial cell line commonly used in cancer research and drug development

references: https://www.atcc.org/products/all/HTB-55.aspx#characteristics