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Fractionation and Centrifugation Word Fill

Niamh C 2 preguntas

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Preguntas del test (2)

  1. 1Fractionation is the process of breaking up cells and separating out their [blank_start]organelles[blank_end]. Before fractionation, the cells need to be under the following conditions:
    1. [blank_start]Cold[blank_end] - to keep the cell alive whilst slowing [blank_start]enzyme[blank_end] reactions
    2. [blank_start]Isotonic[blank_end] - Equal water inside cell and out, which stops o[blank_start]smosis[blank_end] and thus prevents the cell drying up or bursting
    3. [blank_start]pH buffered[blank_end] - to keep enzymes functional by maintaining a constant [blank_start]pH[blank_end]
    1. Aorganelles
    2. BCold
    3. CIsotonic
    4. DpH buffered
    5. Esmosis
    6. Fenzyme
    7. GpH
  2. 2The stages of fractionation:
    1. [blank_start]Homogenisation[blank_end] - the breaking up of cells. Cells are broken up by a homogeniser, which breaks cell membranes and [blank_start]releases[blank_end] organelles.
    2. The cell's contents are now in a solution containing all the organelles (e.g. nuclei, mitochondria, ribosomes.)
    3. Some of the solution is transferred to a test tube.
    4. The test tube is put in a [blank_start]centrifuge[blank_end]
    5. Another tube is put opposite the tube to balance it.
    6. The tubes are spun around a fixed point. This creates a centrifugal, forcing the heaviest organelles to the [blank_start]bottom[blank_end] of the tube.
    7. The [blank_start]pellet[blank_end] produced will contain whole cells, cell wall and nuclei.
    8. The [blank_start]supernatant[blank_end] liquid is transferred into another tube.
    9. This liquid is then centrifuged again. This pellet will contain the next heaviest organelles ([blank_start]mitochondria[blank_end]) and so on (ribosomes etc.)
    1. AHomogenisation
    2. BCoconut
    3. CFractionation
    4. Dreleases
    5. Edestroys
    6. Fcentrifuge
    7. Grefrigerator
    8. Hbottom
    9. Itop
    10. Jmiddle
    11. Kpellet
    12. Lsupernatant
    13. Msupernatant
    14. Npellet
    15. Omitochondria
    16. Pnucleus
    17. Qcell wall
    18. Rretromodernism

Información

Descripción
AS - Level (Cells) Biology Test sobre Fractionation and Centrifugation Word Fill, creado por Niamh C el 27/04/2016.
Autor
Niamh C
Idioma
English
Visitas
11
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