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Protein Function
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- 1CollagenThey are the most common type of fibrous protein found in animals (25-30%). The connect and strengthen tissues, and have a characteristic triple helical structure.
- 2Poly-proline type II characteristicsGlycine as every third residue
Lot of hydroxylated prolines and lysine - 3What hydroxylyzes proline and lysineprolyl hydroxylase
lysyl hydroxylase - 4What causes scurvyA lack of vitamin c > which prevents iron from being reduces > which prevents the function of proly-hydroxylase > proline is not hydroxylised > poly-proline type II doesn't form triple helical structure
- 5Is collagen hydrophobic?Quite hydrophobic due to the many exposed hydrophobic side chains
- 6Ostyogenesis ImperfectaOften glycine mutations.
Prevents proper assembly of triple helix.
Leads to lack of collagen. - 7Malformed or absent boneEhlers–Danlos syndrome (EDS) – a connective tissue disorder caused by a defect in the synthesis of collagen
- 8AntibodiesA specific protein produce by the immune system in response to a foreign molecule (antigen). They bind tightly to these antigens and mark them for destruction.
- 9How are antibodies made?Antibodies are made by B-lymphocytes and are found in blood, lymp and other bodily fluids.
- 10We study Immunoglobulin G or IgGJust know that.
- 11Describe the basic structure of an antibodyThey are made up of 4 chains which are held together by disulphide bonds.
- 12FC region Fragment crystallizedEffector site which usually remains the same across different amino acids.
-activate the complement pathway
-bind to phagocytes
-bind to mast cells, basophils, and eosinophils -bind to NK cells - 132x FAB region fragment antigen bindingThey contain the antigen binding sites
- 14Production of antibodiesCreated by injecting an 'antigen‘ in an animal (often rabbit, goat) After immune response, antibodies harvested from blood.
- 15Uses of antibodiesExtreme specificity makes IgG a valuable tool.
1. Lab experiments: identifying molecules
2. Clinical therapy: Anti venom
3. Clinical: diagnosis of diseases like HIV - 16LigandsMany proteins contain sites to which ligands specifically bind and form a “complex” with the protein. (the site is called the active site on an enzyme)
Binding occurs by multiple weak or a few strong forces
- leads to extreme specificity - 17Ligand binding - Dissociation ConstantKd
High affinity = tight binding = small Kd
Units are moles/L (M)
(Check notes on it - the graphs are great.)
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- 54 (BIOCHEM 2280) Semester 1 Fichas sobre Protein Function , creado por UdaraJay el 25/09/2015.
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- UdaraJay
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