Sample Collection, Processing, and Analysis of Lab Service Options

Description

Ch 2
Nicole Roberts
Flashcards by Nicole Roberts, updated more than 1 year ago
Nicole Roberts
Created by Nicole Roberts over 9 years ago
17
1

Resource summary

Question Answer
Vacutainer tubes contain the appropriate anticoagulant and a vacuum for drawing in the appropriate volume of blood
Red-Top or Serum Collection Tube contains no anticoagulant blood that is placed in this tube is expected to clot so you can get serum
Lavender-Top tubes contains the anticoagulant EDTA salt collect blood for hematologic determinations
Green-Top or Heparin tubes contains lithium heparin advantage is that time is not required for clotting to yield serum the plasma may be separated immediately for testing
Total protein may be slightly higher for plasma because it includes fibrinogen Potassium averages about 0.5mmol/L higher for serum because of platelet release during clotting True
Blue-Top or Citrate Tube contains sodium citrate used for coagulation biochemistry determinations
Sure-Sep Tube variation of the red top tube containing no anticoagulant. stopper is red with black mottling. contains a gel that separates packed cell fractions from serum
Gray-Top or Fluoride Tube contains sodium fluoride (not an anticoagulant) inhibits enzymes in the glycotytic pathway and prevents erythrocytes from metabolizing glucose while whole blood is being transported to the lab not commonly used
Blood collected for a CBC should be analyzed within __ hour or be prepared in the proper way for analysis at a later time 1 hour - if not then blood smear should be prepared and the tube refrigerated
Blood collected in the red top tube is allowed to clot for _____ minutes and then centrifuged to separate the cellular components from the serum 15-30 minutes, serum should be analyzed quickly otherwise refrigerate it for as long as 24-48 hours
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