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Description
Flashcards by Sea Chel Pie, updated more than 1 year ago
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Created by Sea Chel Pie
over 5 years ago
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| Question | Answer |
| Resolution | the minimum distance apart that two objects can be in order for them to appear as separate items |
| Resolution of Light and Electron microscopes? | Light - 0.2 µm Electron - 0.1 nm / 0.0001 µm |
| Magnification | increases the size of an image, does not always increase resolution |
| Magnification of Light and Electron microscopes? | Light - x1500 Electron - x500,000 |
| What is Cell Fractionation? | the process where cells are broken up and the different organelles they contain are separated out |
| Before cell Fractionation, tissue is placed in a Solution which is ; 3 | Cold ❄- reduce enzyme activity, that might break down the organelles Same Water Potential (as Tissue) - prevent organelles bursting or shrinking due to osmotic gain or loss of water Buffered - pH does not fluctuate, which could alter structure of organelles or effect enzyme functioning |
| What is Homogenation? | Cells are broken up by homogeniser - >releases organelles from cell Homogenate (resultant fluid) -> filtered to remove complete cells or large pieces of debris |
| What is Ultracentrifugation? | Fragments in the filtered homogenate are separated in a Centrifuge (which spins tubes of homogenate at very high speeds to create centrifugal force) |
| Ultracentrifugation for animal cells ? | 1. homogenate spun at low speed -> heaviest organelles (nuclei) forced to bottom, forming thin sediment or pellet 2. Supernatant (fluid at top) removed and spun at faster speed -> next heaviest organelles (mitochondria) form sediment 3. process continues with increasing speed and seperated |
| Advances in biological knowledge? | Allowed detailed study or structure and functions of isolated compounds. |
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