469248
Description
Flashcards by emma_moran, updated more than 1 year ago
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Created by emma_moran
about 12 years ago
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| Question | Answer |
| What is the affinity of methylene blue? | Basophilic |
| What structures are stained by methylene blue and what colour are they stained? | Nuclear proteins are stained blue |
| What is the affinity of haematoxylin? | Basophilic |
| What structure are stained by haematoxylin and what colour are they stained? | Nucleic acids: chromatin in the cell nucleus and RNA in the cytoplasm are stained bright orange pink |
| What is the affinity of eosin Y? | Acidophilic |
| What structure are stained by eosin y and what colour are they stained? | Intracellular and extracellular proteins especially in the cytoplasm are stained bright orange pink |
| What is the affinity of Leishman's stain? | Mixed |
| What structures are stained by leishman's stain and what colour are they stained? | Used to identify blood cells. Purple nuclei and granules and pink cytoplasm |
| In a microscope what is the eyepiece and what does is do? | It is a cylinder containing two or more lenses. Its function is to bring the image into focus for the eye. |
| What does the revolving nosepiece do? | Holds multiple objective lenses |
| What do the objective lenses do? | Collect light from the samples |
| In a microscope what is the role of the condenser? | It is a lense designed to focus light from the illumination source onto the sample |
| In a microscope what does the diaphragm do? | Helps to manage the quality and intensity of the illumination |
| What is the limitation of light microscopes? | They can't resolve details of objects that are less than 200nm |
| Why is the resolution of light microscopes limited? | Due to the wavelength of visible light |
| Why do electron microscopes have a greater resolution than light microscopes? | Electrons have a much shorter wavelength than visible light |
| What is the difference between TEM and SEM microscopes? | TEM microscopes produce a 2D image with a very high resolution. SEM produce 3D images with a poorer resolution. |
| How do you prepare a specimen to be viewed under an electron microscope? | The specimen must be completely dried out (dead) because water boils at room temperature in a vacuum |
| Why do electron microscopes contain a vacuum? | Air particles would interfere with the beam of electrons. |
| In electron microscopes why must the specimen be dyed with an electron dense chemical? | Specimens are usually transparent to electrons |
| What is histopathology? | The microscopic examination of cells and tissues for signs of disease |
| How is dye drawn across a slide? | Capillary action |
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Image:
basophil.JPG (image/JPG)
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Basophil- Allergic reaction |
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Image:
eosinophil.JPG (image/JPG)
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Eosinophil- kills parasites |
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Image:
neutrophil (image/jpg)
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Neutrophil- Phagocyte |
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Image:
small_lymphocyte (image/jpg)
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Small lymphocyte- Phagocyte |
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Image:
monocyte (image/jpg)
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Monocyte |
| What are immunostains? | Antibodies designed to label specific proteins or cell structures . The antibodies have fluorescent tags which glow under a fluorescent light source. |
| What is the structure of cheek cells? | Flat for surface covering and they tightly fit together (aggregate) |
| What is the structure of red blood cells? | They are designed for transport. Absences of a nucleus allows maximal cytoplasm space and increases flexibility to pass through blood vessels |
| What is the structure of white blood cells? | Large for phagocytosis |
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