Question | Answer |
DNA profiling | DNA fingerprinting |
DNA profiliing | Making a unique pattern of bands from the DNA of a person |
Step 1 DNA profiling | DNA is released |
Step 2 - DNA profiling | DNA is cut into fragments |
Step 3 - DNA profiling | The fragments are separated |
Step 4 - DNA profiling | Patterns are compared |
Method of amplifying DNA | Polymerase Chain Reaction, PCR |
Enzymes used for cutting enzymes | Restriction Enzymes |
Organism in which restriction enzymes were isolated | Bacteria |
Different restriction enzymes | Cut at different sites |
Example of restriction enzyme | GAATTC restriction enzyme |
Sections of DNA cut out | Restriction Fragments |
Separation of Restriction Fragments | On a basis of size |
Method of separating restriction fragments | Gel Electrophoresis |
Components of gel electrophoresis | Sugar-based gel electric current small glass tank |
Electric current in gel electrophoresis | Draws negatively charged DNA to one end of the gel |
Use of radioactive material in gel electrophoresis | Produce an image of the DNA fragments |
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