Flashcards
HPV Discovery
Cards in this deck (13)
- 1Initial Correlation- Cervical Cancer Found InMarried/widowed women and prostitutes
- 2Initial Correlation- Cervical Cancer Absent InNuns
- 3Initial Correlation- STIs?No correlation found with a particular STI and cervical cancer
- 4Initial Correlation- Why not HPV 11?No known correlation
- 5HPV11 Fishing ToolHPV11 is part of suspected HPV family- so if a HPV strain is involved, it will have a similar genome
- 6DNA Fishing Done BySouthern Blotting
- 7Low StringencyWashing to remove probes done in conditions that allow poorly matched probes to remain bound
- 8High stringencyWashing done in conditions that only allow perfectly matched probes to remain bound
- 9Comparing Low and High StringencyA member of the HPV family would bind with HPV11 at low stringency, but not at high
- 10HPV11 Fishing ResultsWeak binding even at low stringency- not HPV11 but of the HPV11 family
- 11How was HPV16 found?A genomic library- used a nitrocellulose filter to find colonies with HPV DNA to use as a probe
- 12Use of Found HPV as a probeIsolated HPV DNA from samples made into a plasmid for probing
- 13Repeat of Southern BlottingEven under very high stringency, very clear binding- then comapred against all samples, all contained new HPV strain
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- Virology (HPV) Flashcards on HPV Discovery, created by gina_evans0312 on 23/02/2014.
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- gina_evans0312
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