Disaggregation of Explant

Description

Flowchart on Disaggregation of Explant, created by vallarasu VîP on 27/12/2017.
vallarasu VîP
Flowchart by vallarasu VîP, updated more than 1 year ago
vallarasu VîP
Created by vallarasu VîP over 6 years ago
23
0

Resource summary

Flowchart nodes

  • DISAGGREGATION  OF  EXPLANTS
  • Primary explant technique
  • Mechanical diasaggregation
  • Dissection of explant
  • Finely chopped
  • Washed by setting
  • Transfer in last wash
  • REmove BSS
  • Add thin flim of medium
  • Explant out growth
  • Incubate 2 to 3 weeks
  • TISSUE SAMPLE
  • CELL+DEBRIS
  • MORE CELLS+DEBRIS
  • VIABEL CELLS+DEBRIS
  • DILUTE THE CELLS
  • INCUBATE
  • Enzymatic disaggregation
  • Biopsy in medium+antibiotic
  • Dissection in BSS+antibiotic
  • Chopped tissue
  • Complete medium+ collagenase
  • Dissagregated tissue+ tissue residues ​​
  • Dispersed cells+residual culture of epithelial cells​​​​​​
  • Dispersed cell suspension contains enriched fibrobiasts
  • Enriched epithelial fraction
  • Primary cell cul​​​​​​ture
Show full summary Hide full summary

Similar

CPA Exam Sample Questions Pt. 1
nedtuohy
Macromolecules
sealescience
C1:Making Crude Oil Useful (Science-GCSE)
Temi Onas
Haemoglobin
Elena Cade
The Digestive system
Elena Cade
Physics P2
Emmakatewilsonx
GCSE REVISION TIMETABLE
nimraa422
L'environnement
Bryony Whitehead
Contract Law
sherhui94
el centro comercial
Nicholas Guardad
Aplicaciones TIC
Klaudyna Filipkowska