Disaggregation of Explant

Description

Flowchart on Disaggregation of Explant, created by vallarasu VîP on 27/12/2017.
vallarasu VîP
Flowchart by vallarasu VîP, updated more than 1 year ago
vallarasu VîP
Created by vallarasu VîP over 6 years ago
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Resource summary

Flowchart nodes

  • DISAGGREGATION  OF  EXPLANTS
  • Primary explant technique
  • Mechanical diasaggregation
  • Dissection of explant
  • Finely chopped
  • Washed by setting
  • Transfer in last wash
  • REmove BSS
  • Add thin flim of medium
  • Explant out growth
  • Incubate 2 to 3 weeks
  • TISSUE SAMPLE
  • CELL+DEBRIS
  • MORE CELLS+DEBRIS
  • VIABEL CELLS+DEBRIS
  • DILUTE THE CELLS
  • INCUBATE
  • Enzymatic disaggregation
  • Biopsy in medium+antibiotic
  • Dissection in BSS+antibiotic
  • Chopped tissue
  • Complete medium+ collagenase
  • Dissagregated tissue+ tissue residues ​​
  • Dispersed cells+residual culture of epithelial cells​​​​​​
  • Dispersed cell suspension contains enriched fibrobiasts
  • Enriched epithelial fraction
  • Primary cell cul​​​​​​ture
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