Generation of Ureteral Mucosa By Tissue Engineering

Scaffold
1. Cell Cultures: Coculture
  1. hADSC
  2. Urothelial cells
2. Mesh
  1. PLLA
  2. Collagen endolumial stents
In Vitro Assessment
1. MTT: 1,3,5,7 d
2. Optical microscopy and SEM
3. Immunohistochemical staining antycitokeratin 19 antibodies
4. Expresssion of CK-18 and UP2
In vivo Experiments: Grafts in athymical mice
1. 14 days and (2 month, if possible) after implantation
  1. Immunofluorescence: NuMA presence
  2. H&E stainig: cell infiltration
  3. Optical microscopy and SEM
  4. Expression of CK-18 and UP2
Tatiana Fokina, 2A
Bibliografia
1. Ross Histologia texto y Atlas. Correlacion con biologia molecular y celular. Wojciech Pawlina. ISBN 978-84-16004-96-6
2. Recent Advances in Ureteral Tissue Engineering. Paul K. J. D. de Jonge,corresponding author Vasileios Simaioforidis, Paul J. Geutjes, Egbert Oosterwijk, and Wout F. J. Feitz
3. New ureteral scaffold constructed with composite poly(L-lactic acid)–collagen and urothelial cells by new centrifugal seeding system† Authors Wei-Jun Fu, Yong-De Xu, Zhong-Xin Wang, Gang Li, Jian-Guo Shi, Fu-Zhai Cui, Yuanyuan Zhang, Xu Zhang
4. Tissue engineering of ureteral grafts by seeding urothelial differentiated hADSCs onto biodegradable ureteral scaffolds† Jian-Guo Shi, Wei-Jun Fu, Xiao-Xiong Wang, Yong-De Xu, Gang Li, Bao-Fa Hong, Yan Wang, Zhi-Yan Du, Xu Zhang
5. Biomaterials and Tissue Engineering in Urology. J Denstedt, A Atala. ISBN 1845696379, 9781845696375

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