Effect of caffeine on Daphnia heart rate

AD_
Note by AD_, updated more than 1 year ago
AD_
Created by AD_ over 6 years ago
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AS Level AS Biology (IAL AS Biology Unit 3 ) Note on Effect of caffeine on Daphnia heart rate, created by AD_ on 04/23/2014.

Resource summary

Page 1

Independent: Caffeine conc.

Dependent: Daphnia heart rate

Control: Temperature Vol. solution Daphnia stress Daphnia size Acclimatisation time Daphnia species

Method:  Remove Daphnia & place in cavity slide w/ distilled water Leave 5 mins acclimatise, then observe, count heart beat under microscope for 30s x 2 calculate beats/min. Repeats w/ 2 Daphnias Repeat again with diff. conc. caffeine solution. 5 diff conc. = 3 repeats in each conc.

Outcome: Caffeine conc. increased, heart rate increased.

Evaluation Notes: Ensure Daphnia same size If left too long under micropscope, temp increased (due to lamp) = increase heart rate Count heart beat inaccurate High conc. may kill Daphnia Ensure enough data collected

Method

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