Paper Chromatography

Paper Chromatography

1. Mobile phase = molecules can move (liquid or gas)

   • more time spent here = quicker movement through stationary

2. Stationary phase = molecules cannot move (solid or thick liquid)

3. Distance dependent on how soluble they are + how attracted to the paper they are

   • higher solubility + low attraction = more time in mobile = further

4. ink = soluble

5. pencil = insoluble

6. Retention Factor (RF) values

   • Is a certain substance in a mixture
     • Measure pure substance Rf (reference) - compare to Rf spots in mixture
   • RF values = distance each component in the ink moves
     • Each component has a unique RF value
       • Change of solvent = Rf changes
         • Mixture + Reference spot in all solvents is the same = substance present

7. Chriom

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