Totipotency & Tissue Culture

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Method:

Sprinkle seeds on damp sponge & allow germinate.

Use when seeds become sapling.

Make up agar gel into test tube.

With sharp scissors cut tops off below the shoot apex.

Push stem of explants into

the gel, cover with cling film & place

on sunny windowsill.

Observe over 10 days.

Outcome:

Explants

grow roots & leaves continue grow.

Evaluation Issues:

Wrong

part plant cut & inserted into gel.

Unwanted pathogens grow in gel as it is good source of water & nutrients.

Method