Quiz
DNA-Protein Interactions 1
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Questions in this quiz (56)
1What is required for the protein surface to bind to the DNA surface?
- AFor the protein surface to be complimentary to the DNA surface
- BFor the protein surface to be positive (DNA backbone's are negative)
- CFor the protein surface to contain hydrophobic residues
2In which groove can the order of base pairs be differentiated?
- AMajor groove
- BMinor groove
3The major groove is 15A wide
- ATrue
- BFalse
4Which is smaller?
- AMajor groove
- BMinor groove
5Proteins that bind in the major groove must be more specific than those that bind in the minor groove
- ATrue
- BFalse
6Why is Arg such a useful residue?
- AIt has 2 H acceptors, so it can bind to guanine
- BIt has 2 H donors, so it can bind to guanine
- CIt has 2 H acceptors, so it can bind to cytosine
7Which of the following can bind to both G & A
- AGlu
- BVal
- CAsn
- DTyr
8What is base stacking?
- AWhere hydrophobic bases stack to avoid water
- BWhere hydrophobic bases pull the DNA inwards to avoid water
- CWhere hydrophobic bases twist the DNA to avoid water
9What is required for a protein to take advantage of base stacking?
- AResidues with delocalised electrons
- BHydrophobic residues
- CHydrphillic residues
- DFor it to make a gap between bases
1010bp covers a distance of 34 A
- ATrue
- BFalse
11Describe DNA
- ALong, repetitious and thin
- BLong, non-repetitious and thick
- CShort, repetitious and thin
- DLong, repetitious and thick
- EShort, non-repetitious and thin
12DNA can bend 180 dgrees over how long a stretch?
- A40
- B30
- C20
13What does Phage Lamda Repressor bind to?
- APhage DNA
- BBacterial DNA
14What is being shown here?
- AA half site
- BA palindromic sequence
- CA restriction site
15How does Phage Lambda Repressor Work?
- ABinds to bacterial DNA at the lambda cro gene
- BBinds to phage DNA at the Lambda cro gene
- CAnd blocking RNA polymerase binding
16Phage Lambda Repressor binds to only one target sequence
- ATrue
- BFalse
17The Phage Lambda repressor binds as a _
- AMonomer
- BDimer
- CTetramer
18Each subunit attaches to the DNA using a _ and binds to the other subunits using a _
- AAlpha helix, alpha helix
- BAlpha helix, nothing- it's a monomer
- CBeta sheet, beta sheet
- DBeta sheet, nothing- it's a monomer
19Phage Lambda Repressor has multiple subunits because ...
- AIt binds to a sequence long enough that the protein cannot be removed
- BIt binds to a sequence long enough that the sequence becomes unique int the DNA
- CIt binds more exactly - so it only has a single target sequence
20Where does Phage Lambda Suppressor bind?
- A2 adjacent major grooves
- B2 adjactent minor grooves
- CAn adjactent major & minor groove
- D32A apart
- E34A apart
- F36A apart
21A non-specific interaction doesn't involve bonding with any particular base
- ATrue
- BFalse
22List some specific interactions between Phage Lambda Repressor and DNA
- ASer - G, and Gly - T,
- BGly- T and Ser-G
- CGly- C- and Ser-T
23The DNA sequence is all read from the same DNA strand
- ATrue
- BFalse
24A helix-turn-helix motif is often used for reading DNA
- ATrue
- BFalse
25Different alpha helices (of the helix-turn-helix) motif perform specific and non-specific interactions
- ATrue
- BFalse
26Proteins don't have to land on the DNA in exactly the right spot, they only need to...
- ABind to the phosphate backbone
- BBind to the bases
- CAnd then roll freely along the DNA
27Methionine repressor...
- Aprevents methionine production
- Bprevents binding of methionine to tRNA
- Cprevents methionine being added to the amino acid chain
28The Methionine Repressor binding site is...
- APalindromic
- BAlmost palindromic
- C8bp
- D16bp
29The binding site of most helix-turn-helix proteins are 16-18bp long
- ATrue
- BFalse
30How does the Methionine repressor bind to the DNA?
- AUses 2 alpha helices
- BUses 2 beta sheets
- COne from each monomer
- DOne from each end of the monomer
- EInto one major groove
- FInto two major grooves
31Two beta strands have the same diameter as an alpha helix
- ATrue
- BFalse
32Specific interactions in the methionine repressor happen between Lysine, Threonine, A & G
- ATrue
- BFalse
33Restrictions enzymes are part of the bacterial immunity... how?
- ARecognises and destroys non-self DNA
- BCuts around viral DNA that has infected the genome
- CProteases break down the viral coat
34Restriction enzymes and transferases come in pairs, with the restriction enzyme made first
- ATrue
- BFalse
35How does EcoRI bind?
- APhosphate backbone reactions in the minor groove
- BPhosphate backbone reactions in the major groove
- CSpecific interactions in the minor groove
- DSpecific interactions in the major groover
36EcoRI looks for 2 G-C on top of each other because they're more bendable
- ATrue
- BFalse
37Where in the GAATTC sequence does EcoR1 cut?
- AAfter the C, before the C
- BAfter the A & T
- CIn the middle
38Eco RI needs an Fe iron to bind
- ATrue
- BFalse
39Where are the distortions of the DNA caused by EcoRI?
- AIn the centre of GAATTC
- BG/AATT/C
- CGA/AT/TC
40Hydrophobic interactions in ECORI-DNA ineractions recognise what?
- AAA atacking
- BAT stacking
- CTT stacking
41ECORI uses water mediated interactions to assist DNA recognition
- ATrue
- BFalse
42What is strange about the EcoRV restriction enzyme?
- AIt has an abnormally large binding site
- BIt leaves blunt ends
- CIt binds to 2 different sequences
43EcoRV forms a dimer
- ATrue
- BFalse
44ECORV creates major distortions where?
- AIn the middle
- BAt the ends
- COne residue in
45ECORV binds to the backbone in the minor groove and wraps 'arms' around into the major groove
- ATrue
- BFalse
46The recognition loops in the major groove cause...
- AH bonding between stacks
- BH bonding between A-G
- CH bonding between T-C
47ECORV looks for ATAT bases
- ATrue
- BFalse
48Where in the GCGC sequence does M.Hal methylate?
- AThe first G
- BThe first C
- CThe second G
- DThe last C
49Methylation prevents the restriction enzyme from recognizing a sequence, so it cannot be cot
- ATrue
- BFalse
50Describe the actions of methylases
- AMethylate both strands at once
- BMethylate one strand at a time
- CAre monomers
- DAre dimers
51Interactions 'pull out' one of the bases to gain chemical access
- ATrue
- BFalse
52What is the donor for methylation?
- AS-adenosyl-methionine
- BMethanol
- CMethide
53How the methylation interaction studied?
- AThe cystine had the 5th H replaced with an F
- BTrapping it in the active site
- CThe cystine had the 3rd H replaced with an F
54Once in the active site, a thiol bond forms between the cystine and the cytosine so the methyl can be added
- ATrue
- BFalse
55What is inserted to bind with the widowed G?
- AGlu
- BArg
- CCys
56The inserted bases fill up the space left by the flipped out cytosine
- ATrue
- BFalse
Information
- Description
- Sequence Specific & Antiviral Innate Immunity
- Author
- gina_evans0312
- Language
- English
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