ART

1. 1Which of these occur right at ooctye activation?

   1. Acortical granule exocytosis
   2. Bcell cycle resumption
   3. Crecruitment of maternal mRNA
   4. Dcell division
   5. Epronuclei fusion
2. 2At fertilization, the first polar body will be released.

   1. ATrue
   2. BFalse
3. 3At fertilization, the second polar body will be released.

   1. ATrue
   2. BFalse
4. 4The key sperm factor for egg activation is believed to be [blank_start]PLC-zeta[blank_end].

   1. APLC-zeta
5. 5The first calcium comes from [blank_start]in[blank_end]ternal stores. The second comes from [blank_start]ex[blank_end]ternal stores.

   1. Ain
   2. Bex
6. 6Internally stored calcium comes from the

   1. Agolgi
   2. BER
   3. Cmitochondria
7. 7[blank_start]STIM1[blank_end] causes the second calcium wave.

   1. ASTIM1
8. 8Full name. [blank_start]Maturation promoting factor[blank_end] holds the egg in metaphase 2.

   1. AMaturation promoting factor
9. 9An increase in (spell out) [blank_start]calcium[blank_end] activates (abbrev) [blank_start]APC[blank_end] to degrade MPF.

   1. Acalcium
   2. BAPC
10. 104 ways to induce a calcium increase for artificial oocyte activation are el[blank_start]ectropration[blank_end], c[blank_start]alcium ionophores[blank_end], [blank_start]thimerosal[blank_end], e[blank_start]thanol[blank_end].

    1. Aectroporation
    2. Balcium ionophores
    3. Cthimerosal
    4. Dthanol

11. 11Artificial oocyte activation is necessary for nuclear transfer.

    1. ATrue
    2. BFalse
12. 12In mice, which of these can trigger multiple calcium oscillations

    1. ASrCl2
    2. BSiCl2
    3. CSoCL2
    4. DSeCL2
13. 13For artificial oocyte activation, a Ca2 increase isn't the only thing that can be done. Depletion of [blank_start]ZN2+[blank_end] is also an option.

    1. AZN2+
14. 14ICSI stands for [blank_start]intracytoplasmic sperm injection[blank_end].

    1. Aintracytoplasmic sperm injection
15. 15One problem with SCNT is the [blank_start]second polar body release[blank_end], which will often make SCNT embryos [blank_start]haploid[blank_end]. This can be blocked by treating the embryos with [blank_start]cytochalasin B[blank_end] or [blank_start]latrunculin A[blank_end].

    1. Asecond polar body release
    2. Bhaploid
    3. Ccytochalasin B
    4. Dlatrunculin A
16. 16The first AI was done in the [blank_start]dog[blank_end].

    1. Adog
17. 17The first successful ET was done in the [blank_start]rabbit[blank_end].

    1. Arabbit
18. 18PGD stands for [blank_start]preimplantation genetic diagnosis[blank_end].

    1. Apreimplantation genetic diagnosis
19. 19Which of these didn't go through much mouse testing before being used in humans?

    1. AICSI
    2. BKaryotyping
    3. CSperm Sexing
    4. DCytoplasmic transfer
20. 20Artificial activation is needed after ICSI if the sperm [blank_start]can[blank_end] penetrate and [blank_start]fails[blank_end] after that.

    1. Acan
    2. Bcan not
    3. Cfails
    4. Dworks naturally
21. 21The 4 ways an embryo can be sexed by embryo biopsy are [blank_start]X-linked[blank_end] dosage measurement, [blank_start]karyo[blank_end]typing, [blank_start]PCR[blank_end]-based assays, and [blank_start]FISH[blank_end] analysis.

    1. AX-linked
    2. Bkaryo
    3. CPCR
    4. DFISH
22. 22FISH stands for [blank_start]Fluoresence In Situ Hybridization[blank_end].

    1. AFluorescence In Situ Hybridization
23. 23X bearing sperm contain [blank_start]2.8[blank_end]-[blank_start]4.2[blank_end]% more DNA.

    1. A2.8
    2. B4.2
24. 24Sperm are sexed by [blank_start]flow cytometry[blank_end] with a [blank_start]fluorescent DNA[blank_end] dye.

    1. Aflow cytometry
    2. Bfluorescent DNA
25. 25The first cytoplasmic transfer was done in the [blank_start]mouse[blank_end].

    1. Amouse
26. 26[blank_start]Heteroplasmy[blank_end] is the term meaning 2 different mitochondrial DNAs.

    1. AHeteroplasmy
27. 27Faults of the mitochondria never leads to disease.

    1. ATrue
    2. BFalse
28. 28Cytoplasmic transfer is banned in the US.

    1. ATrue
    2. BFalse
29. 29The methods of genetic engineering are r[blank_start]etroviral vector[blank_end]s, p[blank_start]ronuclear microinjection[blank_end], [blank_start]ES[blank_end] cells, [blank_start]PGC[blank_end]s, [blank_start]Sperm-mediated[blank_end] gene transfer, and [blank_start]SCN[blank_end]T. Abbreviate where possible.

    1. Aetroviral vector
    2. Bronuclear injection
    3. CES
    4. DPGC
    5. ESperm-mediated
    6. FSCN
30. 30[blank_start]Pronuclear microinjection[blank_end] involves injecting several copies of a gene, with no targeted modification.

    1. APronuclear injection
31. 31Retroviruses have

    1. ARNA
    2. BDNA
32. 32The size limit for retroviral vectors is [blank_start]5[blank_end]-[blank_start]6[blank_end]kb.

    1. A5
    2. B6
33. 33Which of these is most difficult to reproduce?

    1. ASCNT
    2. Bsperm-mediated gene transfer
    3. Cretroviral vectors
34. 34ES cells are available in m[blank_start]ouse[blank_end], r[blank_start]at[blank_end], h[blank_start]uman[blank_end], and [blank_start]zebrafish[blank_end].

    1. Aouse
    2. Bat
    3. Cuman
    4. Dzebrafish
35. 35Feeder cells for ES cells are [blank_start]irradiated[blank_end] so they don't divide.

    1. Airradiated
36. 36To use ES cells for genetic engineering, the cells must be [blank_start]transfected[blank_end] first. Then they can be injected into blastoceol cavity.

    1. Atransfected
37. 37Embryonic germ cells are derived from [blank_start]PGC[blank_end]s.

    1. APGC
38. 38One advantage of EG cells over ES cells is their high rates of [blank_start]HR[blank_end] (abbrev).

    1. AHR

Description

Lecture 18 & 19

Author

lydiaw4

Language

English

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